样品制备对转基因抗虫棉外源bt蛋白定量检测的影响

《样品制备对转基因抗虫棉外源bt蛋白定量检测的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、棉花学报CottonScience2015袁27渊2冤院184―188样品制备对转基因抗虫棉外源Bt蛋白定量检测的影响*常丽娟袁刘文娟袁张富丽袁王东袁尹全袁雷绍荣袁郭灵安袁宋君渊四川省农业科学院分析测试中心袁成都610066冤摘要院采用ELISA渊Enzyme-linkedimmunosorbentassay冤方法袁以转Bt基因抗虫棉品种中棉所45渊ZGK9822冤和鄂抗虫棉1号渊GK19冤为研究材料袁对叶片的不同组织尧同一样品不同的冻存时间和同一样品不同研磨程度的Bt蛋白进行定量检测遥结果表明院叶片不同组织Bt蛋白表达量差异较小袁叶脉Bt蛋白表达量略高于叶肉和叶柄

2、的Bt蛋白表达量曰转基因抗虫棉单位鲜物质质量Bt蛋白含量和单位可溶性总蛋白Bt蛋白含量随着冻存时间的延长而降低袁鄂抗虫棉1号单位鲜物质质量Bt蛋白含量冻存90d样品与冻存30d尧60d样品差异显著袁单位可溶性总蛋白Bt蛋白含量无显著差异曰不同研磨程度对转基因抗虫棉单位鲜物质质量Bt蛋白含量和单位可溶性总蛋白Bt蛋白含量都存在影响袁其中差异最大的为鄂抗虫棉1号袁200目试样比50目试样单位鲜物质质量Bt蛋白含量上升了56%袁单位可溶性总蛋白Bt蛋白含量上升了38%遥关键词院转基因抗虫棉曰样品制备曰Bt蛋白含量曰定量检测曰ELISA中图分类号院S562.035.3文献标

3、志码院A文章编号院1002-7807渊2015冤02-0184-05院10.11963/issn.1002-7807.201502012*ChangLijuan,LiuWenjuan,ZhangFuli,WangDong,YinQuan,LeiShaorong,GuoLing'an,SongJun(610066,)InordertoevaluatetheeffectsofsamplepreparationonquantitativedetectionofBtproteiningeneticallymodifiedcotton,severalfactors,sucha

4、sdifferenttissuesampledfromleaves,differentfrozenstoragetimeofthesamesamplesanddiffer-entparticlesizeaftergrinding,werestudiedinthisworkusingthetransgeniccottoncultivarsZGK9822andGKl9byELISA.TheresultsshowedthecontentofBtproteininveinwasslightlyhigherthanthatinmesophyllandinpetiole;Btp

5、roteincontentperunitfreshmassweightandperunittotalsolubleproteindecreasedwiththeextensionoffrozentime.SignificantdifferenceswereobservedinBtproteincontentperunitfreshmassweightbetweensampleswithfrozenstoragefor90daysandinsampleswithfrozenstoragefor60daysor30days,whilenodifferencewasfou

6、ndinBtproteincontentperunittotalsolubleproteina-mongsampleswithfrozenstoragefor30,60and90days;TherewasdistinctimpactonBtproteincontentperunitfreshmassweightandperunittotalsolubleproteininsampleswithdifferentparticlesizes.Comparedwithsampleswith50meshinsize,Btproteincontentperunitfreshm

7、assweightinsampleswith200meshinsizeincreasedby56%andBtproteincontentinunittotalsolubleproteinincreasedby38%.transgenicBtcotton;samplepreparation;Btproteincontent;quantitativedetection;ELISA随着转基因技术在棉花育种领域的应用袁转法尧基因芯片法尧ELISA渊Enzyme-linkedimmuno-Bt基因抗虫棉的种植面积不断扩大袁培育的转基sorbentassay冤检测法尧免疫印