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1、中国水稻科学(ChinJRiceSci),2015,29(4):373G381http://www.ricesci.cnDOI:10.3969/ji.ssn.1001G7216.2015.04.006373杂交粳稻亲本米质性状配合力的分子标记区段检测刘洋刘二宝曾思远普伟刘强明梁利君刘佩睿张琪梦洪德林∗(南京农业大学作物遗传与种质创新国家重点实验室,南京210095;∗通讯联系人,EGmail:delinhong@njau.edu.cn)IdentificationofMolecularMarkerFragmentsAss
2、ociatedwithCombiningAbilityforQualityTraitsinParentsofHybridjaponicaRice(OryzasativaL.)LIUYang,LIUErGbao,ZENGSiGyuan,PUWei,LIUQiangGming,LIANGLiGjun,LIUPeiGrui,ZHANGQiGmeng,HONGDeGlin∗(StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUni
3、versity,Nanjing210095,China;∗Correspondingauthor,EGmail:delinhong@njaue.duc.n)LIUYang,LIUErbao,ZENGSiyuan,etal.Identificationofmolecularmarkerfragmentsassociatedwithcombiningabilityforqualitytraitsinparentsofhybridjaponicarice(OryzasativaL.).ChinJRiceSci,2015,29(
4、4):373G381.Abstract:Inordertoimprovericequalitycombiningabilityofparentallinesmoreefficientlyinhybridjaponicarice(OryzasativaL.),SSRmolecularmarkerfragmentgenotypesofelitecombiningabilityforeightqualitytraitswereidentifiedbyanalyzingthedataofcombiningabilityandSS
5、Rmarkersinelevencytoplasmicmalesterile(CMS)linesandninerestorerlines.Theeightqualitytraitsinvestigatedwerebrownricerate(BRR),milledricerate(MRR),headricerate(HRR),percentageofchalkygrain,chalkinessdegree,alkalispreadingvalue(ASV),gelconsistency,andamylosecontents
6、.The11CMSlinesand9restorerlinesweregenotypedusing152pairsofSSRprimers.Combiningabilityanalysiswasconductedusingthedataof99F1smadewithNCIIgeneticdesign(11CMSlines×9restorerlines).Atotalof49SSRmolecularmarkerfragmentgenotypesinvolving27molecularmarkerfragmentsweres
7、ignificantlyassociatedwithgeneralcombiningabilityofthe8qualitytraits.31SSRmolecularmarkerfragmentgenotypeson14chromosomearmswereassociatedwithelitecombiningabilityforqualitytraitsofparents;theremaining18molecularmarkerfragmentgenotypeswereassociatedwithunfavorabl
8、ecombiningabilityofqualitytraitsofparents.Amongthe49molecularmarkerfragmentgenotypes,twowerecoGassociatedwithfourqualitytraitsofparents,andtwowereeachcoGassoci