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1、中国水稻科学(ChinJRiceSci),2013,27(5):473-481http://www.ricesci.cnDOI:10.3969/ji.ssn.1001G7216.2013.05.004473杂交粳稻亲本产量性状优异配合力的标记基因型检测刘二宝1刘洋1刘晓丽1刘强明1赵凯铭1,2EDZESIWisdomMawuli1洪德林1,∗(1南京农业大学作物遗传与种质创新国家重点实验室,南京210095;2江苏省泰州市红旗种业有限公司,江苏泰州225311;∗通讯联系人,EGmail:delinhong@njau.edu.cn)DetectingMar
2、kerGenotypeswithEliteCombiningAbilityforYieldTraitsinParentsofHybridjaponicaRiceLIUErGbao1,LIUYang1,LIUXiaoGli1,LIUQiangGming1,ZHAOKaiGming1,2,EDZESIWisdomMawuli1,HONGDeGlin1,∗(1StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity,Nanjing210095,Chi
3、na;2HongqiSeedCorporation,Taizhou225311,China;∗Correspondingauthor,EGmail:delinhong@njaue.duc.n)LIUErbao,LIUYang,LIUXiaoli,etal.Detectingmarkergenotypeswithelitecombiningabilityforyieldtraitsinparentsofhybridjaponicarice.ChinJRiceSci,2013,27(5):473G481.Abstract:Thekeyfactortospeed
4、inguphybriddevelopmentistoenhancecompetitiveheterosisinjaponicarice,whichdependsonimprovingthecombiningabilityforyieldtraitsoftherestorers.Inordertoimprovecombiningabilityofrestorerlinesmoreefficiently,SSRmarkergenotypeswithelitecombiningabilityfor12traitsweredetected.Thecombining
5、abilitywasanalyzedandSSRmarkersweregenotypedin11MSlinesand9restorerlinesusing152pairsofSSRprimers.Combiningabilityofthe20parentallineswasanalyzedforheadingdate,plantheight,paniclenumberperplant,paniclelength,numberofspikeletsperpanicle,numberoffilledgrainsperpanicle,seedsettingrat
6、e,grainlength,grainwidth,grainthickness,1000Ggrainweightanddailyyieldperunitareausingthedataof99F1smadewithNCIIgeneticdesign.Resultsshowedthat1004SwasthebestMSline,andLR5,YanhuiR50,LC64weresuperiorrestorerlinescomprehensively.Atotalof112SSRmarkergenotypesweresignificantlyassociate
7、dwithcombiningabilityofthe12traits.Amongthem,30wereforonetrait,14werefortwotraits,6wereforthreetraits,3wereforfourtraitsand3wereforfivetraits.RM215G170/180wasassociatedwithseventraits.Markergenotype,RM2439G150/170,wasassociatedwithcombiningabilityofdailyyieldperunitarea,whichincre
8、ased32.6%ofF1grainyield.Keywords: