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ID:9031839
大小:2.89 MB
页数:9页
时间:2018-04-15
《基于srap标记的伊犁河谷野生杏群体遗传多样性研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、园艺学报,2016,43(10):1980–1988.ActaHorticulturaeSinica1980doi:10.16420/j.issn.0513-353x.2016-0156;http://www.ahs.ac.cn基于SRAP标记的伊犁河谷野生杏群体遗传多样性研究1,21232,*李明,胡霞,苗兴军,许正,赵忠12(福建农林大学林学院,福州350002;西部环境与生态教育部重点实验室,西北农林科技大学,陕西杨凌712100;3新疆伊犁哈萨克自治州林业科学研究院,新疆伊宁835000)摘要:利用SRAP分子标记方法对伊犁河谷14个野生杏种群,212份种
2、质资源的遗传多样性进行了研究。结果表明,12对SRAP引物共扩增出条带143条,其中122条具有多态性,多态性比率为85.31%。伊犁河谷野生杏仍然维持较高的遗传多样性水平(h=0.2411,I=0.3708,PPB=85.31%),吐尔根乡种群遗传多样性指数最高。伊犁河谷野生杏存在较高水平的种群内遗传变异(76.42%)和较低水平的种群间遗传变异,种群间存在温和稳健的基因流(Nm=1.3680)。UPGMA系统聚类和遗传结构分析均显示,伊犁河谷野生杏可划分为以霍城样本为主的类群Ⅰ,以巩留和伊宁样本为主的类群Ⅱ和以新源样本为主的类群Ⅲ,种群间遗传距离和地理距离存在
3、具有统计学意义的相关性(r=0.2634,P<0.05)。关键词:杏;遗传多样性;SRAP标记;伊犁河谷;遗传结构中图分类号:S662.2文献标志码:A文章编号:0513-353X(2016)10-1980-09GeneticDiversityAnalysisofWildApricot(Prunusarmeniaca)PopulationsintheIliValleyasRevealedbySRAPMarkers1,21232,*LIMing,HUXia,MIAOXing-jun,XUZheng,andZHAOZhong12(CollegeofForestry,F
4、ujianAgricultureandForestryUniversity,Fuzhou350002,China;KeyLaboratoryofEnvironmentandEcologyinWesternChinaofMinistryofEducation,NorthwestA&FUniversity,Yangling,Shaanxi3712100,China;AcademyofForestry,YiliKazakAutonomousPrefectureofXinjiang,Yining,Xinjiang835000,China)Abstract:Thegenet
5、icdiversityof14wildapricotpopulationswhichinclude212apricotgermplasmsintheIliValleyweredetectedusingSRAPmarkers.Theresultsshowedthat12primersamplificationout143bands,122bandsarepolymorphic,asaratioof85.31%.ThewildapricotsintheIliValleystillmaintainedarelativelyhighlevelofdiversity(h=0
6、.2411,I=0.3708,PPB=85.31%)andpopulationsTuwiththehighestdiversity.Arelativelyhighintraspecificgeneticdiversity(76.42%)andlowinter-populationgeneticdifferentiationwasidentifiedwiththemoderategeneflowvalue(Nm=1.3680).TheUPGMAandgeneticstructureanalysisbothshowedthat14wildapricotpopulation
7、scanbedividedintopredominantlyHuochengsamplesinsubgroupⅠ,predominantlyGongliuandYiningsamplesinsubgroupⅡandpredominantlyXinyuansamplesinsubgroupⅢ,andthereissignificantcorrelation收稿日期:2016–06–15;修回日期:2016–10–17基金项目:国家林业局林业公益性科研专项(200904020);福建省教育厅科技项目(JA15153)*通信作者Authorforcorresponden
8、ce(E-
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