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1、中国水稻科学(ChinJRiceSci),2014,28(2):148-154http://www.ricesci.cn148DOI:10.3969/ji.ssn.1001G7216.2014.02.006利用InDel和SSILP标记分析北方粳型超级稻的遗传组成刘丹#王嘉宇#孙健唐亮陈温福∗(沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室/北方超级粳稻育种教育部重点实验室,沈阳110866;#共同第一作者;∗通讯联系人,EGmail:wfchen5512@126.com.cn)Genet
2、icComponentsAnalysisonSuperjaponicaRiceUsingInDelandSSILPMolecularMarkersLIUDan#,WANGJiaGyu#,SUNJian,TANGLiang,CHENWenGfu∗(RiceResearchInstitute,ShenyangAgriculturalUniversity/KeyLaboratoryofNortheastRiceBiology,GeneticsandBreedingofMinistryofAgriculture/K
3、eyLaboratoryofNorthernjaponicaSuperRiceBreeding,MinistryofEducation,Shenyang110161,China;#Theseauthorscontributedequallytothispaper;∗Correspondingauthor,EGmail:wfchen5512@126c.omc.n)LIUDan,WANGJiayu,SUNJian,etal.Geneticcomponentsanalysisonsuperjaponicarice
4、usingInDelandSSILPmolecularmarkers.ChinJRiceSci,2014,28(2):148G154.Abstract:Geneticbackgroundof15superGriceandtheir21pedigreeGrelativevarieties(including12cultivarsreleasedfrom1977to1999innortheastand9japanesevarietiesbredfrom1963to2000)wererevealedbasedon
5、89subspecificGdifferentiationmolecularmarkers(34InDeland55SSILP).Theresultsshowedthatindicalinagehavealreadyintrogressedintothegenomesof15superGricevarietiesatdifferentlevelwhichrepresentedbyindicaGtypefrequency(F).TheF,varietiesbredin2005-2011wereiivariat
6、ionofsuperGricevarietiesreflectedregistrationperiodsdifferencethehighest(Fi=0.068),followedby1977-1999(Fi=0.033)andrarelyin1963-2000(Fi=0.011).Consequently,indicaGallelefrequenciesofrecentvarietiesweregraduallyincreased.Andthisfactdemonstratesthatacertainp
7、roportionofindicalinageintroducingisanimportantstrategytotheincreasedyieldofnorthernjaponicarice.Keywords:superjaponicarice;indicabloodrelationship;geneticcomponents;subspecificGdifferentiationmolecularmarker刘丹,王嘉宇,孙健,等.利用InDel和SSILP标记分析北方粳型超级稻的遗传组成.中国水稻科学
8、,2014,28(2):148G154.摘要:为明确籼稻血缘对北方粳型超级稻育种的贡献,利用89对籼粳特异性引物(34对InDel和55对SSILP)对15份粳型超级稻品种以及与其系谱密切相关的21份材料(12份1977-1999年育成的常规粳稻品种和9份1963-2000年育成的日本品种)的遗传组成进行分析.结果表明,15份粳型超级稻品种均含有一定的籼型血缘,且不同地区超级稻品种所含籼型基因频率(Fi)有所不同