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1、中国科技论文在线http://www.paper.edu.cnThecompletemitogenomeandgeneorganizationofthe#Chinesewaterdragon(Physignathuscocincinus)**5YANLong,YANPengCollegeofLifeSciences,AnhuiNormalUniversity,AnhuiProvincialKeyLaboftheConservationandExploitationofBiologicalResources,Wuhu241000;10Abstract:
2、ThecompletemitochondrialDNAsequenceoftheChinesewaterdragon(Physignathuscocincinus)wasdeterminedusingPCRandsequencedwithaprimerwalkingmethod.Thecompletemitochondrialgenomeis16,451bpinlength,whichcontains13protein-codinggenes,22tRNAgenes,2rRNAgenesandonecontrolregion(CR).Thegenea
3、rrangementandcompositionofP.cocincinuswassimilartomostothervertebrate,buttheorderofthetRNA-IleandtRNA-Glnwasswitchedand15lackoftheoriginofL-strandreplication(OL).Meanwhile,therewasonlyoneCRinP.cocincinusmitogenome,whereitwaslocatedbetweenthetRNA-ProandtRNA-Phegene.Duplicationof
4、thecontrolregionwasnotfoundinP.cocincinus,whichwasoftenobservedinthemitogenomesofAgamidae.Keywords:Physignathuscocincinus;mitogenome;geneorganization200IntroductionThemitochondrionconstitutesanidealmodelforstudyingevolutionduetogenome[1]rearrangement.Structuralandfunctionalchar
5、acterizationsofthemitochondrialgenomeshavebeenconsideredusefulforphylogeneticstudyinSquamata,suchasgenerearrangements,genetic25codechanges,simplificationoftRNAandrRNAstructures,peculiarmechanismsinreplication[2]-[3]-[4]andtranscription,andbasecompositionalbias.Agamidaeisoneofth
6、elizardfamiliesinSquamata,about480membersofwhicharewidely[5]distributedinthetropicalandsubtropicalregionsofAfrica,AsiaandAustralia.TheChinesewaterdragonbelongstothekingdomAnimalia,phylumChordata,classReptilia,orderSquamata,30suborderLacertilia,infraorderIguania,familyAgamidae,s
7、ubfamilyAgaminae,genusPhysignathusandspeciesPhysignathuscocincinus(Cuvier1829).ThegeneralcharacteristicsofP.cocincinusare:longsnout,moderatebodyinjuvenilesandrobustandcompressedinadults,smalldorsalscalesanduniform,femoralporesinmales(eightpairs),abrightgreendorsumchangeabletobr
8、ownish-green,awhiteventerwithbrightbluepatches,andasno
