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1、BiochemicalSystematicsandEcology39(2011)826–833ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoEstimationofgeneticdiversityinsomeIranianwildPrunussubgenusCerasusaccessionsusinginter-simplesequencerepeat(ISSR)markersa,*a,
2、*abAliShahi-Gharahlar,ZabihollahZamani,RezaFatahi,NaserBouzariaDepartmentofHorticulturalSciences,FacultyofAgriculture,UniversityofTehran,Karaj31587-77871,IranbSeedandPlantImprovementInstitute,HorticultureSection,Karaj,IranarticleinfoabstractArticlehistory:AsIranisoneofthemainoriginsofPrunusger
3、mplasm.Inthisstudy,ISSRmarkerswereReceived13February2011usedforgeneticdiversityevaluationof39accessionsofsubgenusCerasusbelongingtosixAccepted27July2011speciesi.e.PrunusaviumL.,PrunuscerasusL.,PrunusmahalebL.,PrunusincanaPall.,PrunusAvailableonline25August2011microcarpaBoiss.,andPrunusbrachype
4、talaBoiss..With12ISSRprimers,151polymorphicbandsweredetectedwithpolymorphismratiorangeof81.8%–100%.ThelowestsimilarityKeywords:(0.04)wasfoundbetweenP.aviumandP.microcarpagenotypesandthemeanofsimilarityGeneticvariationbetweenallgenotypeswas0.28.ClusteranalysisseparatedimprovedcultivarsfromwildG
5、ermplasmaccessions.ImprovedcherrycultivarsandrootstockswereplacedclosertotheP.aviumConservationISSRthantheotherspecies.Theprincipalcoordinateanalysis(PCoA)supportedtheclusterBreedinganalysisresults.Thewildaccessionswereseparatedaccordingtotheirspeciesandcollectionsites.ISSRmarkersareusefultech
6、niquesforgeneticdiversityevaluationinPrunussubgenusCerasus.Ó2011ElsevierLtd.Allrightsreserved.1.IntroductionSubgenusCerasusbelongstotheRosaceaefamily,Prunoideaesubfamily,andPrunusgenus(Linnaeus,1753).Sweetandsourcherries(PrunusaviumL.,2n¼2x¼16andPrunuscerasusL.,2n¼2x¼32,respectively)arethetwom
7、ainspeciesoftheCerasussubgenuswhichareusedcommercially.Sweetcherryiscultivatedforitsediblefruitandwoodandsourcherryiscultivatedforitssourandsucculentfruit.SpeciesofCerasussubgenusaremainlydistributedinthetemperateandcoolclimatezoneofthe
