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1、BiochemicalSystematicsandEcology39(2011)71–78ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityandrelationshipofendangeredplantMagnoliaofficinalis(Magnoliaceae)assessedwithISSRpolymorphismsaa,*b
2、aHua-HuiYu,Zhi-LingYang,BoSun,Ruo-nanLiuaResearchInstituteofSubtropicalForestry,ChineseAcademyofForestry,FuyangCity,ZhejiangProvince311400,ChinabDanghuHighSchool,PinghuCity,ZhejiangProvince314200,ChinaarticleinfoabstractArticlehistory:Twenty-eightpopulationsoftheraremedicinalplant
3、Magnoliaofficinalis(Magnoliaceae)Received25May2010weresampledacrossitsnaturalrange,andinter-simplesequencerepeats(ISSRs)markersAccepted4December2010wereusedtoassessthegeneticvariationwithinandamongpopulations.Twelveprimercombinationsproducedatotalof137unambiguousbandsofwhich114(83.
4、2%)werepolymorphic.M.officinalisexhibitedarelativelylowgeneticdiversityatpopulationlevelKeywords:(thepercentageofpolymorphiclociPPB¼49.8%,Nei’sgeneticdiversityH¼0.194,ISSRShannon’sinformationindexI¼0.286).However,thegeneticdiversityatspecieslevelwasMagnoliaofficinalisrelativelyhigh(
5、PPB¼83.2%;H¼0.342;I¼0.496).ThecoefficientofgenedifferentiationGeneticdiversityGeneticrelationship(GST,42.8%)andtheresultsofanalysisofmolecularvariance(AMVOA)indicatedthatgeneticdifferentiationoccurredmainlywithinpopulations.Theestimatedgeneflow(Nm)fromGSTwas0.669.Itindicatedthatthef
6、ragmentationandisolationofpopulationsmightresultfromspecificevolutionaryhistoryandanthropogenicactivity.GeneticdriftplayedamoreimportantrolethangeneflowinthecurrentpopulationgeneticstructureofM.officinalis.Conservationstrategiesforthisrarespeciesareproposedbasedonthegeneticdata.Ó2010
7、ElsevierLtd.Allrightsreserved.1.IntroductionGeneticdiversityandrelationshipstudiesaidindevisingpragmaticconservationstrategiesandformulatingcorecollectionsforgeneticresourcesofendangeredplantspecies(JoshiandDhawan,2007),theyarealsocriticalforadaptationtoenvironmentalchangesandforl
8、ong-termsurvivalofaspecies(Venkat
