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1、Availableonlineatwww.sciencedirect.comApplicationofhyperthermophilesandtheirenzymes1,21,21,2HaruyukiAtomi,TakaakiSatoandTamotsuKanaiEnzymesfromhyperthermophilesdisplayextremeexplored.Theaccumulationof(meta)genomesequences(thermo)stabilityandawiderangeofenzymesha
2、vebeeninthedatabasesandthetoolstosearchfordesiredexaminedtoexploretheirpotentialforvariousbiotechnologicalsequences,alongwiththeeasetosynthesizegenesforprocesses.Inaddition,recentyearshavewitnessedtheexpressioninconventionalhostcellsprovidesuswiththedevelopmento
3、fgeneticsystemsinanumberofmeanstoexaminealargenumberofgeneproducts.Thishyperthermophilicarchaea.Thishasprovidedthemeanstoapproachononehandwillnotleadtothediscoveryofinitiatecellengineeringstudiesintheseorganisms.Biofuelstructurallynovelenzymes,butallowsarapidsea
4、rchforproductionisnowanimportanttopicinmicrobialenzymesthatexhibitdifferencesinselectivitytowardsbiotechnology,andthehydrogenproducingcapabilitiesofaparticularsubstrateorstabilitytowardsacertain(hyper)thermophiles,aswellastheirthermostableparametersuchastemperat
5、ure,pHorsolvents.Varioushydrogenases,arenowattractingmuchattention.methodsforhighthroughputactivityandgeneexpressionAddressesscreeninghavealsobeendeveloped[2–5],andcanbe1DepartmentofSyntheticChemistryandBiologicalChemistry,Graduateusedforbothenzymeengineeringand
6、structurallynovelSchoolofEngineering,KyotoUniversity,Kyoto615-8510,Japanenzyme2JST,discovery.CREST,Sanbancho,Chiyoda-ku,Tokyo102-0075,JapanCorrespondingThoroughandinformativereviewsonenzymesfromauthor:Atomi,Haruyuki(atomi@sbchem.kyoto-u.ac.jp)hyperthermophilesan
7、dtheirapplicationareavailableintheliterature[6–10].AmongtherecentprogressonCurrentOpinioninBiotechnology2011,22:618626hydrolyticenzymes,crystalstructuresofesterasesfromThisthehyperthermophilicbacteriumThermotogamaritimareviewcomesfromathemedissueonTissue,celland
8、pathwayengineering[11]andthehyperthermophilicarchaeonPyrobaculumEditedbyUweT.BornscheuerandAliKhademhosseinicalidifontis[12]havebeenreported.Theformerstructurereveale
