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1、JBA-06412;NoofPages21BiotechnologyAdvancesxxx(2011)xxx–xxxContentslistsavailableatScienceDirectBiotechnologyAdvancesjournalhomepage:www.elsevier.com/locate/biotechadvResearchreviewpaperHarnessingthepowerofenzymesforenvironmentalstewardshipPhilippeDemarche,CharlesJunghann
2、s,RakeshR.Nair,SpirosN.Agathos⁎Earth&LifeInstitute,LaboratoryofBioengineering,UniversitéCatholiquedeLouvain,PlaceCroixduSud2/19,1348Louvain-la-Neuve,BelgiumarticleinfoabstractAvailableonlinexxxxEnzymesareversatilecatalystswithagrowingnumberofapplicationsinbiotechnology.T
3、heirpropertiesrenderthemalsoattractiveforwaste/pollutanttreatmentprocessesandtheirusemightbeadvantageousKeywords:overconventionaltreatments.Thisreviewhighlightsenzymesthataresuitableforwastetreatment,withaPollutantbiodegradationfocusoncell-freeapplicationsorprocesseswith
4、extracellularandimmobilizedenzymes.BiologicalwastesareBiocatalysistreatedwithhydrolases,primarilytodegradebiologicalpolymersinapre-treatmentstep.OxidoreductasesLaccaseandlyasesareusedtobiotransformspecificpollutantsofvariousnature.Examplesfrompulpandpaper,Peroxidasetextil
5、e,foodandbeverageaswellaswaterandchemicalindustriesillustratethestateoftheartofenzymaticCellulaseLipasepollutiontreatment.ResearchdirectionsinenzymetechnologyandtheirimportanceforfuturedevelopmentDirectedevolutioninenvironmentalbiotechnologyareelaborated.Besidebiological
6、andbiochemicalapproaches,i.e.enzymeEnzymeimmobilizationprospectionandthedesignofenzymes,thereviewalsocoverseffortsinadjacentresearchfieldssuchasStabilizationinsolubilizationofenzymes,reactordesignandtheuseofadditives.Theeffectivenessofenzymaticprocesses,Bioreactorespecial
7、lywhencombinedwithestablishedtechnologies,isevident.However,onlyalimitednumberofenzymaticfieldapplicationsexist.Factorslikecostandstabilityofbiocatalystsneedtobeaddressedandthecollaborationandexchangebetweenacademiaandindustryshouldbefurtherstrengthenedtoachievethegoalofs
8、ustainability.©2011ElsevierInc.Allrightsreserved.Contents1.Introduction................................