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1、大鼠急性肺损伤分析论文【摘要】目的观察K252a（MLK3阻断剂）对内毒素性急性肺损伤大鼠肺病理组织学改变及生存率的影响。方法采用尾静脉注射脂多糖（lipopolysaccharide，LPS）复制内毒素性急性肺损伤模型。90只大鼠随机分为6组（n=15）：对照组(control组)、LPS组、K252a50μg/kg组（K50组）、K252a75μg/kg组（K75组）、K252a100μg/kg组（K100组）和溶剂对照组（DMSO组）。模型制备成功后6h取肺脏进行光镜检查，观察48h内大鼠死亡情况并比较累计生存率。结果48h内LPS组、K50
2、组、K75组和K100组以及DMSO组大鼠生存率分别为6.3%、27%、72%、57%及6.7%。光镜下可见LPS组大鼠肺组织结构明显被破坏，主要表现为肺泡内出血、肺间质水肿、肺泡萎陷以及肺组织内大量炎性细胞浸润。与LPS组相比，预先应用K252a组肺组织的损伤程度较轻微、肺间隔轻度增宽、无明显出血、少量炎性细胞浸润，尤其K75组，减轻程度最明显。结论K252a延迟LPS大鼠死亡时间，并呈现剂量依赖性，75μg/kgK252a剂量可以明显提高其生存率。并可改善内毒素性急性肺损伤大鼠肺脏的病理组织学结果。【关键词】K252a;MLK3;内毒素;急性肺
3、损伤；大鼠;生存率ProtectiveeffectsofK252aonendotoxin-inducedacutelunginjuryinratsendotoxin-inducedacutelunginjuryinratsZHANGMaoyin1,CHENLong1,CHENGQin1,ZHANGWenwen1,LIUSu2,WANGGuanglei2,LIUGongjian2(1.JiangsuProvinceKeyLaboratoryofAnesthesiology,XuzhouMedicalCollege,Xuzhou,Jiangsu2210
4、02,China;2.DepartmentofAnesthesiology,AffiliatedHospitalofXuzhouMedicalCollege,Xuzhou,Jiangsu221002)6学海无涯Abstract:ObjectiveToinvestigatetheeffectsofK252a(MLK3blocker)onthepathohistologicalchangesinthelungsandthesurvivalrateinratswithendotoxin-inducedacutelunginjury.MethodsRats
5、modelsofacutelunginjurywereestablishedbyadministrationoflipopolysaccharide(LPS)asendotoxininthecaudalvein.90healthymaleSprague-Dawleyratswererandomlydividedinto6groups(n=15):controlgroup,LPSgroup,K252a50μg/kggroup(K50),K252a75μg/kggroup(K75),K252a100μg/kggroup(K100)andvehiclec
6、ontrolgroup(DMSO).Eachgroupwassampled6hafteradministrationofLPSforlightmicroscopyexamination.Thedeathsduring48hineachgroupwereobservedandtheaccumulatedsurvivalrateswerecompared.ResultsWithin48hours,thesurvivalratesofLPSgroup,K50group,K75group,K100groupandDMSOgroupwere6.3%,27%,
7、72%,57%and6.7%,paredwiththecontrolgroup,inLPSgroup,lightmicroscopyrevealedobviousdamagetolungtissues,characterizedbyalveolarhemorrhage,pulmonaryinterstitialedema,paredwithLPSgroup,theK252agrouphadmildhistologicalinjurytolungtissues,withslightthickeningofalveolarseptaandnoevide
8、nthemorrhage,andmildinflammatorycellinfiltration.InK75group,i
