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1、宣肺健脾中药对哮喘大鼠支气管-肺组织病理学和转化生长因子-β1的影响篷劬螓椋笱萌讪揠醌判忱逾蔫去【摘要】 目的观察宣肺健脾中药哮逐平对支气管哮喘大鼠的治疗作用,探讨其可能的作用机制。方法SPF级雄性Wistar大鼠32只,随机分为正常对照组(A组)、哮喘模型组(B组)、地塞米松组(C组)、哮逐平组(D组),每组8只。除A组外,其余组动物建立哮喘模型。HE染色,观察支气管-肺组织病理学改变,以医学图像分析软件测量支气管基底膜周径(Pbm)、支气管壁厚度(Wat)和平滑肌厚度(Wam)。免疫组化PV二步法染色,应用上述图像分析软件检测支气管-肺组织转化生长因子-β1(TGF-β1)的积分光密
2、度(IOD)。结果与A组相比,B组Wat、Wam和支气管-肺组织TGF-β1含量明显增加,差异有统计学意义(P＜0.01)。经药物干预后,C、D组Wat、Wam减少及支气管-肺组织TGF-β1含量显著降低(P＜0.05)。D组Wat、Wam和TGF-β1减少尤为明显,与B组相比,差异有统计学意义(P＜0.05),其中Wat、Wam与A组相比,差异无统计学意义(P＞0.05)。与C组相比,D组TGF-β1含量明显下降,差异有统计学意义(P＜0.01)。结论宣肺健脾中药可能通过抑制哮喘大鼠支气管-肺组织TGF-β1的过度表达而影响平滑肌增殖、减轻气道壁增厚,干预气道重塑。髭鹛茭孪益承啶蛀挹廷
3、国虼喊舾【关键词】 宣肺健脾中药；哮逐平；哮喘；气道重塑；转化生长因子-β1；大鼠鳍砘芰始钧浦袁蘑摘咂洪灼捏骟 Abstract：ObjectiveToobservetheeffectofbronchialasthmainratstreatedbyChuanzhuping,aprescriptionwhichhasthefunctionofdispersinglungQiandinvigoratingspleenQi,andexploreitspossiblemechanism.MethodThirty-twomaleWistarratsofSPFlevelweredividedint
4、ofourgroupsrandomly：controlgroup(A),asthmagroup(B),Dexamethasonegroup(C)andXiaozhupinggroup(D).ExceptgroupA,theotherswereestablishedanimalmodelofasthma.Afterdrawingmaterials,hematoxylinandeosin(HE)stainingwereperformed.Thepathologicalchangesinbronchopulmonarytissueofratsweredetected.Thebasementme
5、mbraneperimeter(Pbm),theairwaywallarea(Wat)andthesmoothmusclewallarea(Wam)weremeasuredandcalculatedbyusingimageanalysissystem.Theconcentrationoftransforminggrowthfactor-β1(TGF-β1)inbronchopulmonarytissuewasobservedbyimmunohistochemicalmethodandtheintegralopticaldensity(IODvalues)ofitwasmeasured.R
6、esultsInbronchopulmonarytissueofgroupB,Wat,WamandtheconcentrationofTGF-β1increased,andweresignificantlydifferentfromthatingroupA(P＜0.01).IngroupCandD,Wat,WamandtheconcentrationofTGF-β1decreased,andtheconcentrationofTGF-β1wassignificantlydifferentfromthatingroupB(P＜0.05).IngroupD,WatandWamweresign
7、ificantlydifferentfromthatingroupB(P＜0.05),andwerenotsignificantlydifferentfromthatingroupA(P＞0.05).IngroupD,theconcentrationofTGF-β1decreasedandwassignificantlydifferentfromthatingroupC(P＜0.01).ConclusionIntreatmentof
