紫外分光光度法测定色氨酸的含量.docx

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1、紫外分光光度法测定色氨酸的含量一：最大吸收波长的测定以0.028mg/ml的标准液进行最大吸收波长的测定，数据记录如下：λ（nm）Aλ（nm）Aλ（nm）A2003.1642660.7713350.0142013.2742670.7863360.0132023.0312680.8023370.0152033.0182690.8153380.0132043.1452700.8253390.0122053.1012710.8323400.0132063.2272720.8333410.0122073.2992730.8313420.0132083.517274

2、0.8333430.0132093.4752750.8363440.0142103.6132760.8453450.0152114.0392770.8523460.0132124.0392780.8573470.0122134.0392790.8553480.0132144.342800.8453490.0122154.2152810.8233500.0122164.342820.7833510.0132174.8172830.743520.0132184.342840.7053530.0122194.1192850.6893540.0112204.342

3、860.693550.0132214.1192870.6943560.0132223.8182880.6733570.0122233.5392890.6193580.012243.2992900.5423590.0112252.8342910.4533600.0122262.3512920.3743610.0112271.8822930.3113620.0112281.4962940.2573630.012291.1792950.2123640.0112300.9522960.1763650.0112310.7752970.1443660.0092320.

4、6472980.123670.012330.5592990.0983680.012340.4883000.0813690.012350.4393010.0663700.012360.4033020.0553710.0092370.3753030.0463720.0092380.3573040.0383730.0092390.3423050.0333740.0082400.3333060.0283750.0092410.3263070.0253760.0082420.3233080.0233770.0092430.3233090.0213780.009244

5、0.3293100.023790.0092450.3343110.023800.0092460.3413120.0193810.0082470.353130.0163820.0082480.3643140.0173830.0082490.3783150.0183840.0092500.3943160.0163850.0092510.4123170.0173860.0082520.4313180.0163870.0092530.4543190.0163880.0092540.4783200.0163890.0092550.5013210.0163900.00

6、82560.5243220.0163910.0082570.553230.0153920.0072580.5733240.0143930.0082590.63250.0153940.0082600.6263260.0143950.0082610.6523270.0143960.0082620.6783280.0143970.0082630.7013290.0133980.0082640.7243300.0143990.0072650.7493310.0154000.0082660.7713320.013　　2670.7863330.013　　2680.80

7、23340.015　　二：吸收线的绘制0.028mg/ml的标准液在200—400nm波长范围内的吸收曲线Aλ（nm）λ（nm）A四：标准曲线的绘制λ（nm）