苦碟子对力竭运动致大鼠心肌损伤时线粒体转录因子A表达的影响.pdf

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1、876中华麻醉学杂志2014年々月第34卷第7期ChinJAnesthesiol，July2014，Vo1．34，No．7．·重症医学·苦碟子对力竭运动致大鼠心肌损伤时线粒体转录因子A表达的影响韩霜何锟赵亚冬容俊芳【摘要】目的评价苦碟子对力竭运动致大鼠心肌损伤时线粒体转录因子A(mtTFA)表达的影响。方法健康雄性Wistar大鼠4O只，体重200～220g，采用随机数字表法分为对照组(c组，=8)、力竭运动组(Es组，n=16)和苦碟子组(Is组，n=16)。采用游泳力竭法建立力竭运动致大鼠心肌损伤模型。Is组于腹腔注射苦碟子注射液20g／kg后行力竭运动。Es组

2、及Is组于力竭后6和24h时分别随机取8只大鼠，采集下腔静脉血样后摘取心脏，采用ELISA法检测血清eTnI浓度，采用Westernblot法检测心肌mtTFA蛋白表达。结果与c组比较，Es组及Is组力竭后6和24h时血清cTnI浓度升高，心肌mtTFA蛋白表达下调(P<0．05)；与Es组比较，Is组力竭后6和24h时血清cTnI浓度降低，心肌mtTFA蛋白表达上调(P<0．05)。结论苦碟子可通过上调心肌mtTFA蛋白表达减轻力竭运动致大鼠心肌损伤。【关键词】菊科；运动过度；心肌疾病；线粒体；转录因子Effectofixerissonchifoliaonmito

3、chondriaitranscriptionfactorAexpressionfollowingmyocardialinjuryinducedbyexhaustingexerciseinratsHartShuang，HeKun，Zhaod0，舶Junfang．DepartmentofAnesthesiology，HebeiGeneralHospital，Shijiazhuang，050000，HebeiProvince，ChinaCorrespondingauthor：RongJunfang，Email：rjf13291@163．con【Abstract】Objec

4、tiveToevaluatetheefectofixerissonchifoliaonmitochondrialtranscriptionfactorA(mtTFA)expressionfollowingmyocardialinjuryinducedbyexhaustingexerciseinrats．MethodsFortymaleWistarrats，weighing200—220g，wererandomlydividedinto3groupsusingarandomnumbertable：controlgroup(groupC，n=8)，exhaustinge

5、xercisegroup(groupES，n=16)andixerissonchifoliagroup(groupIS，n=16)．Themodelofmyocardialinjurywasestablishedbyexhaustingswimming．InISgroup，theratsweresubjectedtoexhaustingswimmingafterintraperitonealixerissonchifolia20g／kg．InESandISgroups，8ratswerechosenat6and24hafterexhaustion，andbloods

6、amplesweretakenfromtheinferiorvenacavafordeterminationofsenlmcardiactroponinI(cTnI)concentrationsbyELISA．TheanimalsweresacrificedafterbloodsamplingandmyocardialspecimenswereobtainedformicroscopicexaminationandfordeterminationofmtTFAexpressionbyWesternblot．ResultsComparedwithgroupC，thec

7、oncentrationofselumcTn1wassignificantlyincreased，andtheexpressionofmtTFAinmyocardialtissueswasdown—regulatedat6and24hafterexhaustioninESand：ISgroups．ComparedwithgroupES，theconcentrationofserumcTn1wassignificantlydecreased，andtheexpressionofmtTFAinmyocardialtissueswasup-regulatedat6an