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1、临床医学论文■流式细胞术检测食管癌细胞DNA含量的临床l=i作者：凌雨田许沈华朱赤红【摘要】目的了解食管癌细胞DNA指数(DI)、S期细胞百分率(SPF)、细胞增殖指数(PI)的临床意义。方法应用流式细胞仪检测64例食管癌手术标本的癌细胞和距癌远端食管切缘粘膜细胞DI、SPF、PI値，并结合随访资料迸行临床病理分析。结果根据DI値，硏究对象分为二倍体组(0.90〜1.10)，亚二倍体组(1・11〜1・20)和异倍体组(>1.20)，分别占53.1%(34/64),21.9%(14/64),25.0%(16
2、/34)。异倍体癌细胞DI、SPF、PI値高于二倍体组(P<0.01)喰管癌异倍体组I〜II期病例占18.8%(3/16),低于二倍体组52.9%(18/34)(P<0.05)。食管癌异倍体组无淋巴结转移病例占25.0%(4/16)，低于二倍体组55.9%(19/34)(P<0.05)。异倍体组与亚二倍体组同时SPF>10%的患者中无淋巴结转移例数占29.4%(5/17)，低于二倍体组无淋巴结转移例数52.9%(18/34)，有统计学差异(P<0.05)。结论食管癌细胞DI値与临床分期、淋巴结转移密切相关
3、。勻食管癌患者生存无显著相关，同时癌细胞的SPF値与淋巴结转移密切相关。【关键词】食管癌DNA指数S期细胞百分率存活率FCM[Abstract]ObjectiveToinvestigatetheclinicalsignificanceofDNAindex(DT),S-phasefraction(SPF),cel1pro]iferationindex(PT)inesophagealcarcinomacelIs・MclhodsDNAcontentanalysisbyFCMwasperformedincance
4、rcellsandmucosalcellsindistalsurgicalmarginisolatedfrom64operativetissuesamplestodeterminetheDT,SPF,PTvaluesinesophagealcarcinomacelIs,informationsonfoilowupwerecombinedtomakeaclinicalpathologicalananlysis・ResultsReserachsubjectsweredividedintothreegroups
5、accordingtoDTvalue:diploidgroup(DI:0.90〜1.10),hypodilpoidorneardiploidgroup(DI:1.11〜1.20),heteroploidgroup(DT>1.20),accountingfor53.1%(34/64),21.9%(14/64),25.0%(16/34)separately.Di,SPF,PIvalueofheteroploidcancercellsweresignificantlyhigherthanthoseofdiplo
6、idcancercells,PvO.Ol.HeteroploidesophagealcarcinomapatientswithstageI〜IIwerein18.8%(3/16),significantly1owerthanthatindiploidpatients,52.9%(18/34),P<0.05・Heteroploidesophagealcarcinomapatientswithoutlymphnodemetastasiswere25.0%(4/16),significanlly1owertha
7、nthatindiploidpatients,55・9%(19/34),P<0.05・Heteroploidesophagealcarcinomapatientswithoutlymphnodemetastasis,harboringmorethan10%SPFwere29.4%(5/17),siginificantly1owerthanthatindiploidpatients,55.9%(19/34),Pv0.05・However,nosignificantdifferencewasfoundbetw
8、eennon・diploidesophagealcarcinomapatientswithoutlymphnodemetastasis,with1owerthant10%SPFanddiploidpatients・Nosignificantdifferenceswerefoundcamparinghypodiploidorneardiploidcancercel1swithdiploidorheteroploidcancerc
