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1、流式细胞术检测食管癌细胞DNA含量临床意义作者：凌雨田许沈华朱赤红【摘要】目的了解食管癌细胞DNA指数(DI)、S期细胞百分率(SPF)、细胞增殖指数(PI)的临床意义。方法应用流式细胞仪检测64例食管癌手术标本的癌细胞和距癌远端食管切缘粘膜细胞DI、SPF、PI值，并结合随访资料进行临床病理分析。结果根据DI值，研究对象分为二倍体组(0.90〜1.10),亚二倍体组(1.11〜1.20)和异倍体组(>；1.20),分别占53.1%(34/64),21.9%(14/64),25.0%(16/34)o异倍体癌细胞DI、SPF、PI值高于二倍体组(P<；0.01)
2、。食管癌异倍体组I〜II期病例占18.8%(3/16),低于二倍体组52.9%(18/34)(P<；0.05)o食管癌异倍体组无淋巴结转移病例占25.0%(4/16),低于二倍体组55.9%(19/34)(P<；0.05)o异倍体组与亚二倍体组同时SPF>；10%的患者中无淋巴结转移例数占29.4%(5/17),低于二倍体组无淋巴结转移例数52.9%(18/34),有统计学差异(P<；0.05)o结论食管癌细胞DI值与临床分期、淋巴结转移密切相关。与食管癌患者生存无显著相关，同时癌细胞的SPF值与淋巴结转移密切相关。【关键词】食管癌DNA指数S期细胞百
3、分率存活率FCM【Abstract】ObjectiveToinvestigatetheclinicalsignificanceofDNAindex(DI),S-phasefraction(SPF),cellproliferationindex(PI)inesophagealcarcinomacells・MethodsDNAcontentanalysisbyFCMwasperformedincancercellsandmucosalcellsindistaisurgicalmarginisolatedfrom64operativetissuesamplestodeter
4、minetheDI,SPF,PIvaluesinesophagealcarcinomacells,informationsonfollowupwerecombinedtomakeaclinicalpathologicalananlysis・ResultsReserachsubjectsweredividedintothreegroupsaccordingtoDIvalue：diploidgroup(DI：0.90〜1.10),hypodilpoidorneardiploidgroup(DI：1.11〜1.20),heteroploidgroup(Dl>；1.20)
5、,accountingfor53.1%(34/64),21.9%(14/64),25.0%(16/34)separately.Di,SPF,PIvalueofheteroploidcancercellsweresignificantlyP<；0.01.withstagelowerthanP<；0.05.higherthanthoseofdiploidcancercells,HeteroploidesophagealcarcinomapatientsI〜IIwerein18.8%(3/16),significantlythatindiploidpatients,
6、52.9%(18/34),Heteroploidesophagealcarcinomapatientswithoutlymphnodemetastasiswere25.0%(4/16),significantlylowerthanthatindiploidpatients,55.9%(19/34),P<；0.05.Heteroploidesophagealcarcinomapatientswithoutlymphnodemetastasis,harboringmorethan10%SPFwere29.4%(5/17),siginificantlylowerthan
7、thatindiploidpatients,55.9%(19/34),P<；0.05.However,nosignificantdifferencewasfoundbetweennon-diploidesophagealcarcinomapatientswithoutlymphnodemetastasis,withlowerthant10%SPFanddiploidpatients・Nosignificantdifferenceswerefoundcamparinghypodiploidorneardiploidcancercellswithdi
