萎胃康颗粒逆转慢性萎缩性胃炎的p53机制研究_程艳.pdf

《萎胃康颗粒逆转慢性萎缩性胃炎的p53机制研究_程艳.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、辽宁中医杂志2013年第40卷第10期·2155·萎胃康颗粒逆转慢性萎缩性胃炎的p53机制研究12程艳，赵岩(1．滨州市人民医院，山东滨州256603;2．滨州医学院，山东烟台264003)摘要:目的:探讨萎胃康颗粒逆转慢性萎缩性胃炎(CAG)的机制。方法:将90只SD大鼠随机抽取18只为正常对照组(正常组)，其余72只采用多重刺激6周复制大鼠CAG模型。确定造模成功的64只随机分为4组，即模型对照组、萎胃康大剂量组、萎胃康小剂量组、三九胃泰组，分别ig生理盐水、萎胃康高浓度水煎液、萎胃康低浓度水煎液和三九胃泰混悬液，1次/d。给药30d后，观察对大鼠胃黏膜

2、组织形态及突变型p53表达的影响。结果:模型组大鼠胃黏膜病理无明显改善，突变型p53表达较正常组显著升高(P＜0．01);各治疗药物均可显著改善胃黏膜萎缩，降低胃黏膜突变型p53表达，较模型组具有显著性差异(P＜0．01)。其中以萎胃康大剂量组大鼠胃黏膜p53的平均光密度最低，较其他治疗组有显著性差异(P＜0．01)。结论:萎胃康能改善和逆转实验性萎缩性胃炎大鼠胃黏膜萎缩，其疗效与剂量有关，其机制可能与抑制细胞的异常增殖有关。关键词:萎胃康;萎缩性胃炎;p53中图分类号:R573．32文献标志码:B文章编号:1000－1719(2013)10－2155－02

3、Researchonp53MechanismofWeiweikangReversingChronicAtrophicGastritis12CHENGYan，ZHAOYan(1．BinzhouPeople＇sHospital，Binzhou256603，Shandong，China;2．BinzhouMedicalUniversity，Yantai264003，Shandong，China)Abstract:Objective:TodiscussthemechanismofWeiweikangreversingchronicatrophicgastritis．

4、Methods:18ratsfrom70SDratswererandomlyselectedoutasnormalcontrolgroup(normalgroup)．Theremaining72ratsweremadeCAGmodelwiththemultiplestimulationsfor6weeks．Theremaining64ratswhichweremodeledsuccessfullywererandomlydividedintofourgroupssuchasmodelgroup，Weiweikanglargedosegroup，Weiweik

5、angsmalldosegroupandSanjiuWeitaigroup．Thegroupswereinjected0．9%saline，Weiweikanghighpotencydecoction，WeiweikanglowpotencydecoctionandSanjiuWeitaisuspension，onceaday．After30days，thegastricmucosapathologicalchangesandtheexpressionofmutantp53wereobserved．Results:Therewasnosignificantd

6、ifferentiationofgastricmucosapathematologyinmodelgroup．Theexpressionofmutantp53madeasignificantincreasecomparedwithmodelgroup(P＜0．01)．Thetherapeuticdrugsallcouldsignificantlyimprovegastricmucosaatrophyandreducetheexpressionofthemutantp53andcomparedwiththemodelgrouptherewasobviousdi

7、fference(P＜0．01)．Theaverageopticaldensityofmutantp53inWeiweikanglargedosegroupwasminimum，withsignificantdifferencecomparedtotheotherther-apeuticgroups(P＜0．01)．Conclusion:Weiweikangcanimproveandreversethegastricatrophyofexperimentalatrophicgastritisofrats，whichrelatedtodose．Itsmecha

8、nismmayberelatedwithrestra