transwell_guide,细胞小室用法说明.pdf

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1、Transwell®PermeableSupportsSelectionandUseGuideLifeSciencesTableofContentsIntroductionCellandtissueculturetechniquesarebecomingIntroduction............................1increasinglyimportantforbasicandappliedlifeSelectingtheRightTranswellMembranescienceresearch.ThedevelopmentofnewcultureandPoreSize.

2、.........................2vesselsandcellattachmentsubstratesiscurrentlybeingdrivenbytheneedtoproduceanenviron-SelectingtheRightTranswellSystem......4mentthatresemblestheinvivostateascloselyaspossibletoenablethegrowthofspecializedcellUsingTranswellPermeableSupports......5types.Consequently,usingperm

3、eablesupportsHelpfulHints..........................5withmicroporousmembraneshavebecomeaGeneralDirectionsforUse...............6standardmethodforculturingthesecells.ThesepermeablesupportshaveallowedsignificantTechnicalAssistance.....................7improvementsinculturingpolarizedcellssincetheseperm

4、eablesupportspermitcellstouptakeOrderingInformation....................8andsecretemoleculesonboththeirbasalandapicalsurfacesandtherebycarryoutmetabolicPolyester(PET)TranswellInserts.........8activitiesinamorenaturalfashion.Collagen-CoatedTranswell-COLInserts...8Membranefiltershavebeenusedascellgrow

5、thPolycarbonateTranswellInserts..........9substratessincethetransfiltermetanephricinduc-Snapwell™Inserts......................9tionstudiesofGrobstein(Nature,172:860-872;1953).AdaptedovertheyearstoavarietyofcellHTSTranswell-24Systems..............10typesandnumerousapplications,permeablesup-HTSTransw

6、ell-96Systems..............11portstreatedforcellgrowtharenowrecognizedasprovidingsignificantadvantagesoversolid,6,12,and24WellCellCulturePlates.....12impermeablecellgrowthsubstrates.Forepithelialandothercelltypes,theuseofpermeablesupportsinvitroallowscellstobegrownand◗Transwell-COLinsertshaveatrans

7、par-studiedinapolarizedstateundermoreent(whenwet),collagen-treatedPTFEnaturalconditions.Cellulardifferentia-membranethatpromotescellattach-tioncanalsoproceedtohigherlevelsmentandspreadingandallowcellsresult