transwell_guide,细胞小室用法说明

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1、Transwell®PermeableSupportsSelectionandUseGuideLifeSciencesTableofContentsIntroductionCellandtissueculturetechniquesarebecomingIntroduction............................1increasinglyimportantforbasicandappliedlifeSelectingtheRightTranswellMembranescienceresearch.Thedevelopmentofnewculturean

2、dPoreSize..........................2vesselsandcellattachmentsubstratesiscurrentlybeingdrivenbytheneedtoproduceanenviron-SelectingtheRightTranswellSystem......4mentthatresemblestheinvivostateascloselyaspossibletoenablethegrowthofspecializedcellUsingTranswellPermeableSupports......5types.Co

3、nsequently,usingpermeablesupportsHelpfulHints..........................5withmicroporousmembraneshavebecomeaGeneralDirectionsforUse...............6standardmethodforculturingthesecells.ThesepermeablesupportshaveallowedsignificantTechnicalAssistance.....................7improvementsinculturi

4、ngpolarizedcellssincethesepermeablesupportspermitcellstouptakeOrderingInformation....................8andsecretemoleculesonboththeirbasalandapicalsurfacesandtherebycarryoutmetabolicPolyester(PET)TranswellInserts.........8activitiesinamorenaturalfashion.Collagen-CoatedTranswell-COLInserts.

5、..8MembranefiltershavebeenusedascellgrowthPolycarbonateTranswellInserts..........9substratessincethetransfiltermetanephricinduc-Snapwell™Inserts......................9tionstudiesofGrobstein(Nature,172:860-872;1953).AdaptedovertheyearstoavarietyofcellHTSTranswell-24Systems..............10t

6、ypesandnumerousapplications,permeablesup-HTSTranswell-96Systems..............11portstreatedforcellgrowtharenowrecognizedasprovidingsignificantadvantagesoversolid,6,12,and24WellCellCulturePlates.....12impermeablecellgrowthsubstrates.Forepithelialandothercelltypes,theuseofpermeablesupportsi

7、nvitroallowscellstobegrownand◗Transwell-COLinsertshaveatranspar-studiedinapolarizedstateundermoreent(whenwet),collagen-treatedPTFEnaturalconditions.Cellulardifferentia-membranethatpromotescellattach-tioncanalsoproceedtohigherlevelsmentandspreadingandallowcellsresult