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1、6高脂饲料诱导的肥胖大鼠网膜素水平的实验研究(400014重庆，重庆医科大学附属儿童医院，儿童发育疾病研究省部共建教育部重点实验室，儿科学重庆市重点实验室，重庆市（儿童发育重大疾病诊治与预防）国际科技合作基地)［摘要］目的人群调查发现肥胖人群网膜素水平较正常人群低，本研究拟检测正常及肥胖大鼠血清网膜素水平及其基因表达情况。方法40只SD成年雄性大鼠随机分为正常组(N=10)和高脂组(N=30)，分别以普通饲料和高脂饲料喂养。六周后从高脂组选取体重增长最快的20只，从中随机抽取10只继续喂养高脂饲料，12周后两组各剩9只，测量后处死并从心脏取血及留取
2、脂肪组织，采用全自动生化仪ADVIA2400测定血糖及血脂、ELISA检测血清胰岛素及网膜素、RT-PCR检测网膜素mRNA表达水平。结果（1）高脂饲料喂养12周后，高脂组大鼠的体重，体重增加值及肥胖指数均高于正常组(分别为591.71gvs525.98g，286.59gvs211.89g,0.049vs0.027,P均<0.05)；（2）高脂组低密度脂蛋白(0.29mmol/lvs0.20mmol/l)及胰岛素水平(0.85ng/mlvs0.67ng/ml)高于正常组(P<0.05)；（3）高脂组血清网膜素水平(99.68ng/mlvs56.98
3、ng/ml)及网膜脂肪组织网膜素mRNA表达水平(1.25vs1.06)均高于正常组(P均<0.05)。结论首次发现肥胖大鼠其血清网膜素水平及网膜脂肪组织中网膜素mRNA表达水平较正常大鼠显著增高，与人群调查结果不一致。［关键词］网膜素；网膜素mRNA表达；大鼠［中图法分类号］R151.4;R-332;R723.14;R363.1［文献标识码］AIncreasedserumomentinanditsmRNAexpressioninhigh-fatdietinducedobeseratsLvPanpan,QuPing,BiYang,ChenJie,L
4、iuYouxue(Children’sHospitalofChongqingMedicalUniversity,Chongqing400014,China.MinistryofEducationKeyLaboratoryofChildDevelopmentandDisorders.KeyLaboratoryofPediatricsinChongqing,CSTC2009CA5002.ChongqingInternationalScienceandTechnologyCooperationCenterforChildDevelopmentandDis
5、orders)［Abstract］ObjectiveObesepopulationshavesignificantlylowerplasmaomentinlevelsthanleanones,ThepresentstudywastoinvestigatetheserumomentinlevelanditsmRNAexpressioninomentaladiposetissueinnormalandhighfatdiet-inducedobeserats.MethodsMaleadultSprague-Dawleyratswererandomlydi
6、videdinto2groups:10ratsincontrolgroupfedwithnormaldiet,30ratsinexperimentalgroupwithhighfatdiet(HFD).After6weeks,10ratswererandomlychosenfromthetop20ratswiththehighestweightgain.Theexperimentalrats(n=10)werefedwithHFDforanother6weeks.Atendofstudy,bloodsamplesweretakenfromheart
7、forthemeasurementofserumlipids,glucose,insulinandomentin.AdiposetissuesamplesweretakenfordeterminationofthemRNAexpressionofomentinbyRT-PCR.Results(1)After12weeksofhighfatdietfeeding,bodyweight,weightgainandtheobesityindexweresignificantlyhigherinHFDgroupratsthanthoseincontrolg
8、roup(525.98gvs591.71g,211.89gvs286.59g,0.027vs0.049,respectiv
