Salvinorin A对全脑缺血再灌注大鼠海马内皮生长因子表达和脑损伤的影响.pdf

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1、ShanghaiMedJ，2014，Vo1．37，No．8·678·●基础与危重医学●SalvinorinA对全脑缺血再灌注大鼠海马内皮生长因子表达和脑损伤的影响王震虹忻纪华王祥瑞【摘要】目的观察SalvinorinA(SA)对大鼠全脑缺血再灌注(I／R)后海马内皮生长因子(VEGF)表达和脑细胞损伤的影响及其机制。方法成年健康雄性Sprague_Dawley(SD)大鼠4O只，体重300～350g，随机均分为4组：假手术组、I／R组、SA组和Norbin(阿片受体拮抗剂)+SA组。I／R后6h行免疫组织化学检查分析海马CA1、CA3区

2、VEGF蛋白相对表达量，脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测海马CA1、CA3区细胞凋亡，苏木精一伊红染色后在光学显微镜下观察细胞坏死情况。结果I／R后6h，I／R组CA1和CA3区凋亡细胞表达阳性率和坏死细胞比例均显著高于假手术组、SA组和Norbin+SA组(P值均

3、蛋白相对表达量显著高于Norbin+SA组(P值均<0．05)。结论全脑缺血10min再灌注可以导致神经细胞的凋亡和坏死，SA可以减轻全脑缺血引起的脑组织损伤，促进VEGF在海马CA1和CA3区的表达。sA的作用部分是通过阿片受体实现的。【关键词】脑缺血再灌注；SalvinorinA；细胞坏死；细胞凋亡；内皮细胞生长因子EffectofSalvinorinAonexpressionofvascularendothelialgrowthfactorinhippocampusaftercerebralischemia-reperfusion

4、WANGZhenhong，XINJihua，WANGXiangrui．DeportmentofAnesthesiology，RenjiHospital，ShanghaiJiaotongUniversitySchoolofMedicine，Shanghai200127，ChinaCorrespondingauthor：WANGXiangrui[Abstract]ObjectiveToobservetheefectandmechanismofSalvinorinA(SA)ontheexpressionofvascularendothelia

5、lgrowthfactor(VEGF)inhippocampusaftercerebralischemia-reperfusion(I／R)．MethodsMaleSprague-Dawley(SD)ratsweighing300—350gweredividedinto4grouprandomly：shamoperationgroup，I／Rgroup，SAgroup，andNorbin(kappaopioidreceptorantagonist)+SAgroup．TheexpressionofVEGFproteininhippocam

6、pusCA1andCA3regionwasmeasuredbyimmunohistochemistry6hafterI／R．TheapoptosiswasinvestigatedbyTdT-mediateddUTPnickendIabeling(TUNEL)．Cellnecrosiswasidentifiedbypathologicaltest．ResultsThepositivepercentagesofcellapoptosisandnecrosisweresignificantlyhigherinI／ngroupat6hfollo

7、wingischemia-reperfusionthanthoseinshamoperationgroup，SAgroupandNorbin+SAgroup(allP<0．05)．ButthepositivepercentagesofcellapoptosisandnecrosisweresignificantlylowerinSAgroupthanthoseinNorbin+SAgroup(allP<0．05)．UpregulationofVEGFwasshowedinI／Rgroupascomparedwithshamoperati

8、ongroup，furtherupregulationinSAgroupandNorbin+SAgroupinCA1andCA3(allP<0．05)．AndtheexpressionofVEGFinSAg