经皮穴位电刺激对慢性运动性疲劳大鼠的影响.pdf

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1、J．Acupunct．Tuina．Sci．2012，10(5)：265—270D0I：l0．1007／sl1726—012一O617—6SpecialTopicStudyEffectofTranscutaneousElectricalAcupointStimulationonRatswithChronicExercise-·inducedFatigue经皮穴位电刺激对慢性运动性疲劳大鼠的影响FangJian—qiao(方剑乔)，MaGui—zhi(马桂芝)，LiangYi(梁宜)ZhejiangUniversity

2、ofTraditionalChineseMedicine，Hangzhou310053，China【摘要】目的：观察经皮穴位电刺激(TranscutaneousElectricalAcupointStimulation，TEAS)对运动性疲劳大鼠的影响。方法：采用递增负荷跑台法建立大鼠慢性运动性疲劳模型，第6星期开始，分别在运动后即刻和3h后进行TEAS治疗。治疗2星期后，HE染色观察大鼠海马、下丘脑组织形态学变化，免疫组化法观察海马、下丘脑5一羟色胺(5一hydroxytryptamine，5-HT)的表达。结果

3、：TEAS可以明显减轻运动引起的大鼠海马、下丘脑组织形态损伤，降低大鼠海马、下丘脑5-HT表达，运动后即刻治疗和3h后治疗的两组间无统计学差异。结论：TEAS有促进疲劳恢复，提高运动能力的作用。TEAS改善大鼠的运动性疲劳状态与降低中枢5一HT表达、减少神经细胞损伤有关。【关键词】穴位疗法；肌肉疲劳：5一羟色胺：海马；下丘脑：大鼠【中图分类号】R2—03【文献标志码】A【Abstract】objective：Toobservetheeffectoftranscutaneouselectricalacupointst

4、imulation(TEAS)ontheratswithexercise—inducedfatigue．Methods：Ratmodelofchromicexercise—inducedfatiguewasestablishedwithincreasingloadtreadmillwalkingmethod．TheTEAStherapywasoperatedimmediatelyand3haftertheexercisefromthesixthweek．Hippocampalmorphologyandhypotha

5、lamicmorphologywereobservedwithHEstainingandtheexpressionsof5一hydroxytryptaminer5一HT)inhippocampusandhypothalamusweretestedwithimmunohistochemical(IHC)stainingafter2一weektreatment．Results：TEASreleasedthedamageofhippocampalmorphologyandhypothalamicmorphologyind

6、ucedbyexercisesignificantlyandreducedtheexpressionof5一HTinhippocampusandhypothalamus．Thedifferencebetweenthetherapyoperatedimmediatelyand3hafterexercisewasnotsignificant．Conclusion：TEAScouldimprovethefatiguerecoveryandathleticability．TheimprovementofTEASonther

7、atswithexercise—inducedfatiguewasrelatedwiththedecreasedexpressionofcentral5-HTandthereductionofdamagednervecells．[KeyWords】AcupointTherapy；MuscleFatigue；Serotonin；Hippocampus；Hypothalamus；RatsExercise．inducedfatigueisacommondiseaseinTEASonexercise．inducedfati

8、gueandthesportsmedicineandtheresearchontherecoveryofmechanismofthecentral5．hydroxytryptamineexercise—inducedfatigueisoneoftheproblemsto(5一HT)J．Thusweestablishedtheratmodelofbesolve