高糖刺激对H9c2心肌细胞凋亡及miR-34a表达的影响.pdf

《高糖刺激对H9c2心肌细胞凋亡及miR-34a表达的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、2O13。29临床心血管病杂志(10)：785—788JournalofClinicalCardiology(China)·785··II床基石出研究·高糖刺激对H9c2心肌细胞凋亡及miR一34a表达的影响*赵芳Y-4~,芝陈明汪朝晖。汪瀚干学东熊世熙王扬淦[摘要]目的：探讨高糖对H9c2细胞凋亡及表达miR-34a的影响。方法：采用高糖(25mmol／L)刺激H9c2细胞(实验组)，并设空白对照(对照组)。采用Tunel法和流式细胞仪检测细胞凋亡，采用定量PCR检测miR一34a、Bcl一2和Caspase一3基因表达的变化，Westernblotting检测凋亡相关蛋白Bcl

2、一2和Caspase一3的蛋白表达。结果：实验组H9c2细胞凋亡率较对照组明显增加[(10．47±0．21)：(6．21±0．02)，P

3、1[文献标志码]A[文章编号]1001—1439(2013)i0—0785—04EffectsofhighglucoseonapoptosisandmiR一34aexpressionofH9c2cellsZHAOFangWE工YinzhiCHENMingW_ANGZhaohuiW_ANGHanGanXuedongXONGShixiW_ANGYanggan(DepartmentofCardiovascularDisease，ZhongnanHospital，WuhanUniversity，Wuhan，430071，China；DepartmentofCardiology，UnionH

4、ostipal，HuazhongUniversityofScienceandTechnology)Correspondingauthor：ZHAOFang，E—mai1：cedilanid@163．comAbstractObjective：ToinvestigatetheeffectsofhighglucoseontheapoptosisofH9c2cellsandtheexpres—sionchangesofmiR一34aofH9c2cells．Method：H9c2celllineofcardiomyocyteswererandomlydividedintonormalcont

5、rolgroupandhighglucosegroup．Terminal—deoxynucleotidyltransferasemediatednickendlabeling(TUNEL)andflowcytometrywereusedtodeterminehighglucoseinducedapoptosisinH9c2cells．Theexpres—sionofmiR-34awasdetectedbyreal—timePCR．Apoptosis—relatedproteinsBcl一2。Caspase一3weredetectedbythemethodsofRT—PCRandWe

6、sternblotting．Result：Comparedwithnormalcontrolgroup，cellapoptoticrateandtheexpressionofmiR-34awereincreased，themRNAandproteinexpressionofBcl一2weresignificantlydecreased，andtheexpressionofCaspase-3weresignificantlyincreasedinhighglucosegroup(allP

7、ptosisandregulatetheexpressionofapoptosis—relatedmicroRNA—miR一34a．Keywordsdiabetesmellitus；cardiomyocytes；apoptosis；miR一34a糖尿病(diabetesmellitus，DM)是一组以慢性楚_2]。微小RNA(microRNA，miR)广泛表达于血浆葡萄糖水平增高为特征的代谢性疾病群，持续各个组织和器官，其中miR一34a参与多种细胞在不高血糖可以引起