细针穿刺细胞学联合流式细胞术在B细胞非霍奇金淋巴瘤诊断及分类中的应用.pdf

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1、白血病·淋巴瘤2014年4月第23卷第4期JournalofLeukemia＆Lymphoma，April2014，Vo1．23，No．4·223··论著·细针穿刺细胞学联合流式细胞术在B细胞非霍奇金淋巴瘤诊断及分类中的应用卞恒娟王会平陶千山熊术道张翠陶莉莉潘莹翟志敏【摘要】目的探讨细针穿刺(FNA)细胞学(FNAC)联合流式细胞术(FCM)在B细胞非霍奇金淋巴瘤(B—NHL)诊断及分类中的价值。方法对17例经组织病理诊断为B—NHL的患者行FNA，获取穿刺细胞后迅速涂片，经瑞特一吉姆萨染色及显微镜观察细胞形态；同时，制备细胞悬液，标记相应

2、抗体后在流式细胞仪上检测，结果与组织病理诊断比较。结果17例B—NHL患者中，FNAC联合FCM可诊断15例，1例因取材不佳无法诊断，另1例考虑为反应性淋巴组织增生，与病理诊断符合率为93．8％；进一步分类诊断符合率为93．3％。结论在临床高度怀疑为淋巴瘤但无法行病理活组织检查的特殊情况下，FNAC联合FCM可为B．NHL的诊断及分类提供重要的信息。【关键词】淋巴瘤，非霍奇金；细针穿刺术；细胞学；流式细胞术Theutilizationoffineneedleaspirationcytologycombinedwithflowcytometr

3、yindiagnosingandclassifyingB-cellnon-HodgkinlymphomaBianHen~uan,WangHuiping,TaoQianshan，XiongShudao，ZhangCui，TaoLili,PanYing,ZhaiZhimin．AnhuiMedicalUniversityHeamatologicalResearchCenter,theSecondHospitalofAnhuiMedicalUniversity,Bio—MedicalResearchCenter,Hefei230601，ChinaC

4、orrespondingauthor：ZhaiZhimin，Email：zzzm889@163．com【Abstract】0bjectiveToevaluatetheaccuracyoffineneedleaspiration(FNA)cytology(FNAC)combinedwithflowcytometry(FCM)indiagnosingandclassifyingB—cellnon—Hodgkinlymphoma(B—NHL)．MethodsFNAwasperformedon17patientswithhistopathology

5、—diagnosedlymphoma．SomeofFNAmaterialwassmearedonslidesandstainedbyWrightSstain，andthencellmorphologywasobservedundermicroscope．Theleftmaterialwaspreparedtocellsuspensionsandtestedforimmunophenotypewithflowcytometry．Accordingtothe2008WHONHLclassification，theB-NHLwasdiagnose

6、dandclassifiedthroughFNACcombinedwithFCM．Theresultswerecomparedwithhistopathologyresults．ResultsIn17casesofB—NHLwhichwerediagnosedbyhistopathology，15caseswerediagnosedbyFNACcombinedwithFCM．Amongthetwonegativecases，onewasduetoinsuficientmaterialandtheotherwasdiagnosedasreac

7、tivelymphoidtissuehyperplasia．So．thecoincidenceratesofdiagnoseandclassificationwere93．8％and93．3％．ConclusionFNACcombinedwithFCMmayaffordsufficientevidencetodiagnoseandclassifyB—NHL，especiallyforthosecasesthatarehighlysuspiciousforlymphomabutdificulttogethistopathology．【Keyw

8、ords】Lymphoma，non—Hodgkin；Biopsy，fine—needle；Cellcytology；FlowcytometryB细胞非霍奇金淋巴瘤(B—NHL)为