蕨麻正丁醇部位抑制缺氧损伤心肌细胞凋亡及Caspase3／9表达.pdf

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1、第31卷第8期中华中医药学刊V0l_31NO．82013年8月CHINESEARCHIVESOFTRADITIONALCHlNESEMEDICINEAug．2013蕨麻正丁醇部位抑制缺氧损伤心肌细胞凋亡及Caspase3／9表达李灵芝，韦薇，龚海英，李建宇，郭鹏，张永亮，(1．武警后勤学院，天津300162；2．天津市职业与环境危害生物标志物重点实验室，天津300162)摘要：目的：研究蕨麻正丁醇部位对缺氧损伤心肌细胞凋亡及Caspase3、Caspase9表达的抑制作用。方法：建立大鼠原代培养心肌细胞缺氧模型，HE染色观察细胞组织形态，PI

2、—Hochesst3342染色观察细胞凋亡，RT—PCR技术及免疫细胞化学染色方法检测细胞Caspase3、Caspase9mRNA及其产物蛋白水平。结果：与模型组比较，正丁醇部位各剂量组Caspase3、Caspase9mRAN表达水平均显著降低(P<0．05或P<0．01)；高剂量组Caspase3、Caspase9蛋白水平显著降低(P<0．01)。结论：蕨麻正丁醇部位可能通过减弱缺氧诱导的心肌细胞Caspase级联反应，抑制心肌细胞凋亡。关键词：蕨麻；缺氧；细胞凋亡中图分类号：R542．2文献标志码：A文章编号：1673—7717(20

3、13)08—1573—03EfectofN—butanolPotentillaAnserineL．FractiononExpressionsofCaspase3andCaspase9inCardiomyocyteInjuredbyHypoxiaLILingzhi，WEIWei，GONGHaiying，LIJianyu，GUOPeng，ZHANGYongliang，(1．LogisticalUniversityofChinesePeoplegArmedPoliceForces，Tianjin300162，China；2．TianjinKeyLa

4、boratoryofOccupationalandEnvironmentalHazardsBiomarkers，Tianjin300162，China)Abstract：Objective：Tostudytheinhibtingeffectofthen—butanolPotentillaanserineL．fractionontheapoptosisandtheexpressionsofCaspase3andCaspase9ofcardiomyocyteinjuredbyhypoxia．Methods：Todevelopamodelofcar

5、diomycyteinjuredbyhypoxiainratsinvitro．ThepathologychangesandtheapoptosisofcardiomycyteswereobservedseparatelybyHEstainingandPI—Hoechst3342．TheexpressionlevelsofCaspase3andCaspase9mRNAsinthecardio—mycyteswerestudiedbyreversetranscriptionpolymerasechainreaction(RT—PCR)．Thelo

6、calizationsandlevelsoftheirproductproteinsinthecardiomycyteswereinvestigatedbyimmunocytochemistrystain．Rersult：Comparingwithmodelgroup，thepathologychangeswereimprovedbythen—butanolPotentillaanserineL．fractionandthenumberofapoptoticcardiomycyteswasreduced．Aftertreatedwithdif

7、ferentdosesofn—butanolPotentillaanserineL．fraction，theexpres—sionlevelsofCaspase3andCaspase9mRANsincardiomyocytessignificantlydecreased(P<0．05orP<0．01)．Thecontentsoftheirproductproteinssignificantlydecresedinthehighdosegroup(P<0．01)．Conclusion：Then—butanolPotentillaanserine

8、L．fractionexertsaremarkablyinhibitingeffectoncardiomyocyteduringhypoxiaandthismayb