柯萨奇B3病毒对心脏发育因子GATA-4、NKx2.5表达的影响及叶酸对其保护作用.pdf

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1、ll84TiariiinMedJ，Dec2013，Vol41No12doi：lO．3969~．issn．0253—9896．2013．12．013柯萨奇B3病毒对心脏发育因子GATA一4、NKx2．5表达的影响及叶酸对其保护作用薛雅馨仇小强余红平谭盛葵。【摘要】目的探讨叶酸对孕期感染柯萨奇B3病毒(CVB3)的新生鼠心脏发育因子GATA一4、NKx2．5的基因与蛋白表达的影响。方法sD雌鼠分为对照组、叶酸组、CVB3组、CVB3+叶酸组。叶酸组及CVB3+@酸组孕前给予叶酸灌胃2N；CVB3组及CVB3+叶酸组孕第7天腹腔注射CVB3，连续5d，自然分娩后取新生

2、鼠心脏组织，病理切片HE染色观察新生鼠心脏组织形态学变化；逆转录聚合酶链反应(RT—PCR)及WesternblotNI]定新生鼠心脏组织中转录因子GATA一4、NKx2．5基因及蛋白表达水平。结果CVB3组新生鼠心肌损伤明显，心肌细胞排列紊乱，心肌纤维断裂，CVB3+叶酸组状态改善。CVB3组新生鼠心脏组织GATA一4、NKx2．5基因及蛋白表达低于对照组(P<0．05)，CVB3+叶酸组GATA一4、NKx2．5基因及蛋白表达高于CVB3组(P<0．05)。结论孕鼠早期感染CVB3抑制新生鼠心脏发育因子正常表达，补充叶酸对新生鼠心脏发育具有明显保护作用。【关

3、键词】柯萨奇病毒感染；叶酸；心脏缺损，先天性；GATA4转录因子；逆转录聚合酶链反应；印迹法，蛋白质；大鼠，Sprague—Dawley；NKx2．5ProtectiveEfectsofFolicAcidonCardiacDevelopmentRelatedGenesGATA一4andNKx2．5ExpressionofOfspringRatswithMaternaICoxsackievirusB3InfectionXUEYaxin，QIUXiaoqiang，YUHongping，TANShengkuiDepartmentofCardiology,Afiliat

4、edHospitalofGuilinMedicalUniversity,Guilin541000,China；2GuangxiMedicalUniversity；3PublicHealthSchool，GuilinMedicalUniversity[Abstract】ObjectiveToinvestigatetheeffectsoforalfolicacidoncardiacdevelopmentrelatedgeneexpressionofoffspringinanexperimentalmodelofcoxsackievirusB3(CVB3)infect

5、ionofpregnantrats．MethodsSDfemaleratswererandomizedintocontrolgroup，folicacidgroup，CVB3groupandCVB3+folicacidgroup．Thefemaleratsweregivenfolicacidbygavagefor2weeksbeforepregnancyinfolicacidgroupandCVB3+folicacidgroup．Afterconceptionfor7days，ratmodelwasestablishedbyintraperitonealinje

6、ctionofCVB3for5daysinCVB3groupandCVB3+folicacidgroup．Afternat—uralchildbirthneonatalheartwastakenandstoredinliquidnitrogen．ThemorphologicalchangesofneonatalratmyocardialtissueswereobservedbyHEstaining．TheexpressionsofGATA一4andNKx2．5mRNAweredetectedbyRT—PCRandWest—ernblotassay．Results

7、Therewassignificantmyocardialinjury，suchasmyocardialfiberdisarrayandmyocardialfiberbreakage，inneonatalratsinCVB3group．ThesedamageswereimprovedinCVB3+folicacidgroup．TheexpressionlevelsofGATA一4andNKx2．5genesinmyocardialtissuesweresignificantlylowerinCVB3groupthanthoseofcontrolgroupfP<0

8、．051．Theexpr