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《旋毛虫肌幼虫排泄分泌蛋白对H446小细胞肺癌的抑制作用.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国病原生物学杂志2013年12月第8卷第l2期JournalofPathogenBiologyDecember2013,Vo1.8,No.12旋毛虫肌幼虫排泄分泌蛋白对H446·论著·小细胞肺癌的抑制作用*常红敏,关晓东,李丹,赵蕾,罗婧梅,邴玉艳,陈晓宁,杜娈英(1.承德医学院病原生物学教研室,河北承德067000;2.承德石油高等专科学校机械丁程系,河北承德067000)【摘要】目的研究旋毛虫肌幼虫排泄分泌蛋白对H446小细胞肺癌的抑制作用。方法试验随机分为3组。A组(实验组):将提取的旋毛虫肌幼虫排泄分泌蛋白用无血清培养基稀释成浓度为0.075、
2、0.15和0.3mg/ml,分别与H446细胞共培养;B组(标准对照):将顺铂稀释成12.8、6.4、3.2、1.6、0.8、0.4和0.2~g/ml,分别与H446细胞共培养;C组(空白对照组):H446细胞培养过程中不作任何处理。采用噻唑蓝比色法(MTT法)检测各组不同时间细胞抑制率;采用原位末端转移酶标记法(TUNEI法)观察细胞凋亡情况。结果MTT法检测各实验组细胞抑制率均随药物浓度的增高和作用时间的延长而升高,与空白对照组比较差异有统计学意义(P%0.01);TUNEL法检测A、B、c组细胞凋亡率分别为85、43和2,差异有统计学意义(P3、01);A组与B组比较差异有统计学意义(P4、llaspiralisonH446lungcancercellsCHANGHong—min,GUANXiao—dong,LIDan,ZHAOLei,IUOJing—mei,BINGYu—yan,CHENXiao—ning,DULuan—ying(1.DepartmentofPathogenBiologyofChengdeMedicalCollege.Cheng—de,Hebei067000,China2.DepartmentofMechanicalEngineering,ChengdePetroleumCollege)[Abstract]Objectiv5、eThisstudymonitoredtheinhibitoryactionofproteinssecretedbyTrichinellaspiralisonH446smallcelllungcancertoidentifytheantitumoractivityofthoseproteinsintheH446celllineofsmallcelllungcancer.MethodsH446ceilswererandomlydividedintothreegroups:anexperimentalgroup(GroupA),astandardcontro6、lgroup(GroupB),andacontrolgroup(GroupC).InGroupA,extractedsecretoryproteinsofT.spiralisweredilutedwithserumfreemediumandthenaddedtoculturedH446ceilsataconcentrationof0.075mg/ml,0.15mg/ml,and0.3mg/m1.InGroupB,culturedH446cellsweretreatedwithdilutedcisplatinataconcentrationof12.8tL7、g/ml,6.4/~g/ml,3.2>g/ml,1.6/*g/ml,0.8gg/ml,0.4/~g/ml,and0.2~g/m1.InGroupC,culturedH446cellswerenottreated.AnMTTcolorimetricassaywasusedtodeterminetherateofinhibitioninthegroupsofcellsatdifferenttimes.TUNELwasusedtoobserveapoptosis.ResultsTherateofinhibitionwasfoundtoincreaseovert8、imeandastheconcentrationoftheaddedtreatm
3、01);A组与B组比较差异有统计学意义(P4、llaspiralisonH446lungcancercellsCHANGHong—min,GUANXiao—dong,LIDan,ZHAOLei,IUOJing—mei,BINGYu—yan,CHENXiao—ning,DULuan—ying(1.DepartmentofPathogenBiologyofChengdeMedicalCollege.Cheng—de,Hebei067000,China2.DepartmentofMechanicalEngineering,ChengdePetroleumCollege)[Abstract]Objectiv5、eThisstudymonitoredtheinhibitoryactionofproteinssecretedbyTrichinellaspiralisonH446smallcelllungcancertoidentifytheantitumoractivityofthoseproteinsintheH446celllineofsmallcelllungcancer.MethodsH446ceilswererandomlydividedintothreegroups:anexperimentalgroup(GroupA),astandardcontro6、lgroup(GroupB),andacontrolgroup(GroupC).InGroupA,extractedsecretoryproteinsofT.spiralisweredilutedwithserumfreemediumandthenaddedtoculturedH446ceilsataconcentrationof0.075mg/ml,0.15mg/ml,and0.3mg/m1.InGroupB,culturedH446cellsweretreatedwithdilutedcisplatinataconcentrationof12.8tL7、g/ml,6.4/~g/ml,3.2>g/ml,1.6/*g/ml,0.8gg/ml,0.4/~g/ml,and0.2~g/m1.InGroupC,culturedH446cellswerenottreated.AnMTTcolorimetricassaywasusedtodeterminetherateofinhibitioninthegroupsofcellsatdifferenttimes.TUNELwasusedtoobserveapoptosis.ResultsTherateofinhibitionwasfoundtoincreaseovert8、imeandastheconcentrationoftheaddedtreatm
4、llaspiralisonH446lungcancercellsCHANGHong—min,GUANXiao—dong,LIDan,ZHAOLei,IUOJing—mei,BINGYu—yan,CHENXiao—ning,DULuan—ying(1.DepartmentofPathogenBiologyofChengdeMedicalCollege.Cheng—de,Hebei067000,China2.DepartmentofMechanicalEngineering,ChengdePetroleumCollege)[Abstract]Objectiv
5、eThisstudymonitoredtheinhibitoryactionofproteinssecretedbyTrichinellaspiralisonH446smallcelllungcancertoidentifytheantitumoractivityofthoseproteinsintheH446celllineofsmallcelllungcancer.MethodsH446ceilswererandomlydividedintothreegroups:anexperimentalgroup(GroupA),astandardcontro
6、lgroup(GroupB),andacontrolgroup(GroupC).InGroupA,extractedsecretoryproteinsofT.spiralisweredilutedwithserumfreemediumandthenaddedtoculturedH446ceilsataconcentrationof0.075mg/ml,0.15mg/ml,and0.3mg/m1.InGroupB,culturedH446cellsweretreatedwithdilutedcisplatinataconcentrationof12.8tL
7、g/ml,6.4/~g/ml,3.2>g/ml,1.6/*g/ml,0.8gg/ml,0.4/~g/ml,and0.2~g/m1.InGroupC,culturedH446cellswerenottreated.AnMTTcolorimetricassaywasusedtodeterminetherateofinhibitioninthegroupsofcellsatdifferenttimes.TUNELwasusedtoobserveapoptosis.ResultsTherateofinhibitionwasfoundtoincreaseovert
8、imeandastheconcentrationoftheaddedtreatm
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