紫草素对人共刺激细胞杀伤胃癌细胞的影响及其机制.pdf

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2、辱华^诗牝枭童’@世界华人消化杂志2014年5月18B；22(14)：1984—1991wcjd@wjgnet．cornISSN1009—3079(print)ISSN2219—2859(online)研究快报＆AJPIDCOMMUNICATION紫草素对人共刺激细胞杀伤胃癌细胞的影响及其机制郭雪兴，刘军权，陈复兴，吕小婷，陈永强，陈剑群人共刺辫粤胞是量，l陈复：，虽，J壁，陈永强，中国人民解放军第九七医CCK8assaywasusedtossesstl1eproliferation一种由外周血淋院实验科江苏省徐州市221000’_一．⋯

3、一：～：‘二二⋯⋯～⋯⋯巴细胞经7种细胞陈剑群，徐州医学院附属医院消化内科江苏省徐州市otco。snmula【eⅡceils,anqIVlllassaywasusect因q-在体外诱导221000tomeasurethereducedrateofgrowthofgastric麓鐾c。a—ncercells．FCM—was邶useadtoddetecttheexpres-妻霎茎瑟摹氢簇嚣廛咖LDHereleaskeassay∞wasu咖sedtoamceasuretheinHflmu．-雯蕈毒对人釜其刺萎-澍接受日期：2014—04—09在线出

4、版日期：2014—05—18a0s∞saywasusedtom“eansurlep卜J—、cate[n1P-ERK1．／2,增殖和功能Bcl一2andP-AKTexpressioninco—stimulatedcells的影响．bef0reandafterskonininduction．Shikonin．inducesCytotoxiCityofco-stimulatedhumancellsRESULTS：Afterincubationwithshikoninattogastriccancercelllinesconcentrationsra

5、ngingfrom0．2to50pg／Lfor48h，theproliferationrateofco。stimulatedcellsXue-XingGuofJun—QuanLiuFu-XingChenincreasedsignificantlyfP<0．05)．Theexpres—Xiao-TingLv,Yong-QiangChen，Jian-QunChensionofGraB，PFP，CD107a，IFN一丫，p—catenin，P—ERK1／2Bcl一2andP—AKTonco—stimulatedXue-XingGuo，Departm

6、entofGastroenterology,XuzhoucellstreatedwithshikoninatconcentrationsMedica1College，Xuzhou221000，JiangsuProvince．Chinafrom6to25~tg／Lweresignificantlyhigherthan3un—quanLiu，Fu-XingChen，Xiao-TingLv,Yong-QiangChen．DepartmentofCentralLaboratory,97Hospitalofthatinthecontro1groupfP

7、<005)．Inaddition,PLA，Xuzhou221000JiangsuProvince，Chinathecytotoxicactivit、，ofco—stimulatedcellstreat-Jian-QunChen，DepartmentofGastroenterology,A佑liatededwithshikoninagainstthethreegastriccancerHospitalofXuzhouMedicalCollege．Xuzhou221000．Ji—celllinesweresignificantlyhigherth

8、anthatinangsuProvince，ChinaCOrresDondenceto：Jian—QunChen，ChiefPhysician，thecontrolgroupfP<0．05)．DepartmentofGastroenterology,A街liatedHospita1OfXu—zhouMedica1College．99HuanhaiWestRoad．QuanshanCONCLUS10N：Theseresultssuggestthatshi—District．Xuzhou22l000，JiangsuProvince，China．x

9、yfyzzk@163．comkonincanpromotethegrowthofco—stimulatedReceived：2014—03—13Revised：20