脑神康胶囊对糖尿病大鼠记忆能力及海马HIF-1α表达的影响.pdf

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1、第51卷第7期山东大学学报(医学版)2013年7VO1．5lNO．7J0URNALOFSHAND0NGUNIVERSITYfHEALTHSCIENCES)Ju1．20l3文章编号：1671—7554(2013)07—0020—05DOI：10．6040／j．issn．1671—7554．2013．07．005脑神康胶囊对糖尿病大鼠记忆能力及海马HIF—loL表达的影响李旭阳，刘德山，梁尔顺，董玲芬，赵莉，高伟(山东大学齐鲁医院中医科，济南250012)摘要：目的研究脑神康胶囊对糖尿病大鼠记忆能力及海马乏氧诱导因子(HIF—loL)表达的影响。方法将36只大鼠分为正常对照组、糖尿病组、中药治疗组

2、，中药治疗组用脑神康胶囊水溶液灌胃，其余两组用等量生理盐水灌胃，每日1次，持续12周。在0、4、8、12周测血糖及进行Morris水迷宫实验，使用免疫组化及RT—PCR技术检测海马HIF—loL的表达。结果中药治疗组与糖尿病组大鼠HIF一1表达量较正常对照组增高，中药治疗组第8、12周较糖尿病组HIF—l表达量减少(P<0．01或P<0．05)。水迷宫潜伏期自第4周始中药治疗组较糖尿病组缩短(P<0．01或P<0．05)，较正常对照组延长。结论脑神康胶囊能下调HIF一1仪表达，改善记忆能力下降症状，减轻糖尿病脑功能损害。关键词：脑神康胶囊；糖尿病；乏氧诱导因子；海马；水迷宫中图分类号：R285

3、．5文献标志码：AEffectsoftheNaoshenkangcapsuleonmemoryandexpressionofHIF一1OLinhippocampustissueofdiabetesmellitusratsLIXu—yang，LIUDe—shan，LIANGEr—shun，DONGLing—fen，ZHAOLi，GAOWei(DepartmentofTraditionalChineseMedicine，QiluHospitalofShandongUniversity，Jinan250012，China)Abstract：0bjectiveTostudytheeffectofthe

4、Naoshenkangcapsuleonmemoryandexpressionofhypoxiainduciblefactor一1alpha(HIF一1)inhippocampustissueofdiabeticrats．MethodsThirty—sixratsweredividedintothreegroups：thecontrolgroup，thediabeticgroup，andtheNaoshenkanggroup．NaoshenkangcapsulewasadministeredtotheNaoshenkanggrouponcedaily，andanequalvolumeofnor

5、malsalinewasgiventothecontrolandthediabeticgroups．Alloperationscontinuedfor12weeks．Morriswatermazewasoperatedeveryfourweeks．Meanwhile，theex—pressionofHIF一10【inhippocampustissuewasdetectedbyimmunohistochemistryandRT—PCR．ResultsTheexpres—sionofHIF一10【increasedbothinthediabeticandtheNaoshenkanggroups．C

6、omparedwiththediabeticgroup．theNaoshenkanggrouphadanobviousdecreaseintheexpressionofHIF一10lsincetheeighthweek(P<0．01orP<0．05)，anditalsohadareductioninthelatencyperiodofMorriswatermazesincethefourthweek(P<0．01orP<0．05)．ConclusionTheNaoshenkangcapsulecaneffectivelydown—regulatetheexpressionofHIF—lo【an

7、dimprovethememoryability，andfinallyamelioratethebraindamagecausedbydiabetes．Keywords：Naoshenkangcapsule；Diabetesmellitus；Hypoxiainduciblefactor一1alpha；Hippocampus；Morriswatermaze学习记忆是动物认知活动的主要内容，海马组下降