绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力.pdf

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1、Pangeta1．／JZhejiangUniv-SciB(Biomed&BiotechnoO201415(~：533—539JournalofZhejiangUniversity-SCIENCEB(Biomedicine＆Biotechnology)ISSN1673—1581(Print)；ISSN1862—1783(Online)wwwZjUedu．cn~zus；WMPN．springerlinkcomE—mail：jzus@zjueducaGreenteapolyphenol，epigaU0catechin一3一gallate，possessestheantilvilralactivilt

2、ynecessarYytofitig~Zhtag~ainstthehepatitisBvirusreplication／nvitroJing．yaoPANG~Kui-junzHA0§1，Jia-bowANG

3、2，Zhi-jieMA丰1，Xiao．heXIA0，(ClinicalPharmacTLaboratory,BeijingFriendshHospitalCapitalMedicalUniversity,Beo'ing100050,China)(ChinaMilitaryInstituteofChineseMedicine,302MilitaryHospitalBeijing100039,

4、China)(IntegrativeMedicineCenter,302MilitaryHospitalBeijing100039,China)E-mail：pharmsci@126．com；13811647091@163．comReceivedDec．5，2013；RevisionacceptedMar．16，2014；CrosschcckedMay19，2014Abstract：AlthoughseveraIantiviraldrugsandvaccinesareavailableforuseagainsthepatitisBvirusfHBV)，hepatitiscausedbyHBVr

5、emainsamalorpublichealthproblemworldwide．whichhasnotyetbeenresolved．andnewanti-HBVdrugsareingreatdemand．Thepresentstudywasperformedtoinvestigatetheanti-HBVactivityofepigallocatechin．3．gallate(EGCG)，anatura1．origincompound，inHepG22．2．15cells．TheantiviraIactivityofEGCGwasexaminedbydetectingtheIevelsof

6、HBsAgandHBeAginthesupernatantandextracellularHBVDNA．EGCGefectivelysup．pressedthesecretionofHBsAgandHBeAgfromHepG22．2．15cellsinadose—andtime-dependentmanner．anditshowedstrongerefectsattheIeveIof0．11-一0．44pmol／mI(50—200pg／m1)thanIamivudine(3TC)at0．87IJmol／mlf200pg／m1)．EGCGalsosuppressedtheamountofextr

7、acellularHBVDNA．ThedataindicatedthatEGCGpossessedanti．HBVactivityandsuggestedthepotentiaIofEGCGasanefectireanti．HBVagentwithIowtoxicity．Keywords：Epigallocatechin-3-gallate，HepatitisBvirus(HBV)，Antiviralactivity，HepG22．2．15，Lamivudinedoi：10．163l／jzus．B1300307Documentcode：ACLCnumber：R965．11Introductio

8、ninurgentdemand．Long．termHBVinfectionmaydevelopintoHepatitisBcausedbyhepatitisBvirus(HBV)ishepaticfibrosis，livercirrhosis，andhepatocellularamaiorpublichealthproblemthathasnotbeenre—carcinoma，accountin