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时间:2020-05-19
《肾炎防衰液对糖尿病肾病大鼠的防治作用及对于MCP-1表达的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、·482·虫国中西医结合肾病杂志2015年6月第16卷第6期CJITWN,June2015.Vo1.16.No.6肾炎防衰液对糖尿病肾病大鼠的防治作用及对于MCP一1表达的影响孙鲁英①孙卫卫①金化男①李侠①刘玉宁①王耀献①(摘要]目的:研究肾炎防袁液对糖尿病肾病大鼠的防治作用。方法:建立大鼠糖尿痛肾痛模型,随机分为模型组、贝那普利组和肾炎防衰液组,另设假手术组;给予贝那普利组和肾炎防衰液组分别灌胃以贝那普利混悬液(1.0mg·kg~·d)和肾炎防衰液(11.4g·kg。。·d);模型组和假手术组灌以等量生理盐水。实验进入第8周后取材,检测
2、血清肌酐、尿素氮,采用HE和Masson染色检测肾脏病理、免疫组化法检测肾组织MCP一1的表达。结果:各组大鼠血清尿素氮水平差异无统计学意义(P>0.05);模型组血清肌酐水平明显高于假手术组(P<0.05),而肾炎防衰液组较模型组为低(P<0.05);模型组肾小球硬化指数、肾小管损伤指数、肾小管间质纤维化指数以及肾组织中MCP一1表达显著高于假手术组(P<0.05);肾炎防衰液组肾小球硬化指数、肾小管损伤指数、肾小管间质纤维化指数以及MCP一1表达均较模型组为低(P<0.05)。结论:肾炎防衰液可以降低血清肌酐水平,减轻糖尿病肾病大鼠肾
3、小球硬化及肾间质纤维化的程度,下调糖尿病肾病大鼠肾组织McP—l的表达,延缓糖尿病肾痛进展。[关键词]肾炎防衰液糖尿病肾病MCP一1TheEfectoftheShenyanfangshuaiSolutionontheTherapyofDiabeticNephropathyandtheExpressionofMCP——1forSTZ—-inducedRatsSUNLuying,SUNWeiwei,JINHuanan,etalDongzhimenHospitalBeijingUniversityofChineseMedicine,Beijin
4、g(100700)ABSTRACTObjective:TostudytheefectoftheShenyanfangshuaiSolution(theTraditionalChineseMedicalSolutionforpreventingand~eafingrenalfailure)onthetherapyofdiabeticnephropathy(DN)andtheexpressionofMCP一1forSTZ—inducedrats.Methods:Themodelratswiththediabeticnephrepathy(DN
5、)weretreatedbythenephrectomyandintraperhonealinjectionofstreptozotocin.Allmodelratswererandomlydividedintomodelgroup,benazeprilgroupandShenyanfangshuaiSolutiongroup.Thehealthyratswerechosenassham—operationgroup.Allratswereventherelevantsolutionsrespectivelyfor8weeks.Thera
6、tsofbenaseprilgroupweretakenbyintragastricadministrationwiththebenazeprilsuspensionliquid(1.0mg·kg~·d),andtheratsofShenyanfangshumSolutiongroupintragastricadministrationwiththeShenyanfangshuaiSolution(11.4g·kg~·d)respective-ly.Theratsofmodelgroupandsham—operationgroupwere
7、takenbyintragastricadministrationwithphysiologicalsaline.Whenthetrialwasfinished,theserumcreatinineandureani~ogenweredetected.RenalpathologywasdetectedbyHEandMassonstaining.TheexpressionofMCP一1inrenaltissuewasdetectedbyimmnuohistochemistry.Results:Therewasnosignificantdif
8、erenceaboutserumureanitrogenlevelineachgroupofrats(P>0.05).Thevaluesofserumcreatinineinmodelgrou
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