肾炎防衰液对糖尿病肾病大鼠的防治作用及对于MCP-1表达的影响.pdf

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1、·482·虫国中西医结合肾病杂志2015年6月第16卷第6期CJITWN，June2015．Vo1．16．No．6肾炎防衰液对糖尿病肾病大鼠的防治作用及对于MCP一1表达的影响孙鲁英①孙卫卫①金化男①李侠①刘玉宁①王耀献①(摘要]目的：研究肾炎防袁液对糖尿病肾病大鼠的防治作用。方法：建立大鼠糖尿痛肾痛模型，随机分为模型组、贝那普利组和肾炎防衰液组，另设假手术组；给予贝那普利组和肾炎防衰液组分别灌胃以贝那普利混悬液(1．0mg·kg～·d)和肾炎防衰液(11．4g·kg。。·d)；模型组和假手术组灌以等量生理盐水。实验进入第8周后取材，检测

2、血清肌酐、尿素氮，采用HE和Masson染色检测肾脏病理、免疫组化法检测肾组织MCP一1的表达。结果：各组大鼠血清尿素氮水平差异无统计学意义(P>0．05)；模型组血清肌酐水平明显高于假手术组(P<0．05)，而肾炎防衰液组较模型组为低(P<0．05)；模型组肾小球硬化指数、肾小管损伤指数、肾小管间质纤维化指数以及肾组织中MCP一1表达显著高于假手术组(P<0．05)；肾炎防衰液组肾小球硬化指数、肾小管损伤指数、肾小管间质纤维化指数以及MCP一1表达均较模型组为低(P<0．05)。结论：肾炎防衰液可以降低血清肌酐水平，减轻糖尿病肾病大鼠肾

3、小球硬化及肾间质纤维化的程度，下调糖尿病肾病大鼠肾组织McP—l的表达，延缓糖尿病肾痛进展。[关键词]肾炎防衰液糖尿病肾病MCP一1TheEfectoftheShenyanfangshuaiSolutionontheTherapyofDiabeticNephropathyandtheExpressionofMCP——1forSTZ—-inducedRatsSUNLuying，SUNWeiwei，JINHuanan，etalDongzhimenHospitalBeijingUniversityofChineseMedicine，Beijin

4、g(100700)ABSTRACTObjective：TostudytheefectoftheShenyanfangshuaiSolution(theTraditionalChineseMedicalSolutionforpreventingand~eafingrenalfailure)onthetherapyofdiabeticnephropathy(DN)andtheexpressionofMCP一1forSTZ—inducedrats．Methods：Themodelratswiththediabeticnephrepathy(DN

5、)weretreatedbythenephrectomyandintraperhonealinjectionofstreptozotocin．Allmodelratswererandomlydividedintomodelgroup，benazeprilgroupandShenyanfangshuaiSolutiongroup．Thehealthyratswerechosenassham—operationgroup．Allratswereventherelevantsolutionsrespectivelyfor8weeks．Thera

6、tsofbenaseprilgroupweretakenbyintragastricadministrationwiththebenazeprilsuspensionliquid(1．0mg·kg～·d)，andtheratsofShenyanfangshumSolutiongroupintragastricadministrationwiththeShenyanfangshuaiSolution(11．4g·kg～·d)respective-ly．Theratsofmodelgroupandsham—operationgroupwere

7、takenbyintragastricadministrationwithphysiologicalsaline．Whenthetrialwasfinished，theserumcreatinineandureani~ogenweredetected．RenalpathologywasdetectedbyHEandMassonstaining．TheexpressionofMCP一1inrenaltissuewasdetectedbyimmnuohistochemistry．Results：Therewasnosignificantdif

8、erenceaboutserumureanitrogenlevelineachgroupofrats(P>0．05)．Thevaluesofserumcreatinineinmodelgrou