槐米中芦丁超声法和碱溶酸沉法提取比较.doc

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1、2011届　　　　　　　　　分类号：　　　　　　　　　　　　　　　　　单位代码：10452毕业论文槐米中芦丁超声法和碱溶酸沉法提取比较ComparationofUltrasonicandAlkalisolutionandacidprecipitationextractedRutinfromSophorajaponica·1摘要槐花中主要成分为芦丁,又名芸香苷,现在报道的提取方法有很多，本实验主要研究超声提取法与碱溶酸沉法,比较这两种方法提取率高低。超声法是用70%乙醇溶液浸泡槐米粉，在超声清洗器中进行超声处理30min，处理2次，收集滤液，用浓盐酸调节PH

2、值至3，静置，至沉淀完全后，抽滤，收集沉淀得到芦丁粗品，粗品在60-70℃水浴中溶解，煮沸15min，趁热抽滤，静置滤液，待沉淀完全，抽滤，沉淀在65℃干燥40min，得到芦丁的纯化物；碱溶酸沉法是用蒸馏水浸泡，加氧化钙1-1.5g，调节PH至8，加热至微沸，保持40min，趁热过滤，浸煮2次，收集滤液，用浓盐酸调节PH至5，静置至沉淀完全，将沉淀在60-70℃水浴中，溶解，煮沸15min，趁热抽滤，静置滤液，待沉淀完全，抽滤，沉淀在65℃干燥40min，得到芦丁的纯化物。本实验中碱溶酸沉法比超声法的提取率高。关键词：槐米；超声提取法；碱溶酸沉法；紫外分光

3、光度法；聚酰胺薄膜ABSTRACTThisexperimentcomparedultrasonicextractionwithalkalisolutionandacidprecipitationinextractionrateofrutin.UltrasonicmethodissoakedSophorajaponicawith70%ethanol,theultrasoniccleanercarriedouttheSophorajaponicawith30minutes.thenfilteringthesolutionandcarriedouttheresi

4、dueintheultrasoniccleaneragain.CollectingthefiltrateinbeakerandAdjustingthefilter’sPHto3withconcentratedhydrochloricacid.Standingthefilterandprecipitatingcompletely.Collectingtheprecipitatebythefiltration.Theprecipitateiscrudeextractrutin.Recrystallizatingthecrudeextractrutinin60-

5、70℃waterbathandboilingtherecrystallizate’ssolutiononelectricfurnacewith15minutes.Filtratingthesolutionwhenitisboilingandthefiltrateasideuntiltheprecipitatecompletely.Filtratingthesolution,Thencollectingtheprecipitate.Theprecipitatedriedat65℃inVacuumovenwith40minutes.Gettingthepuri

6、fiedrutin.AlkalisolutionandacidprecipitationissoakedSophorajaponicawithdistilledwater,Addingcalciumoxide1.5g.adjustingthePHto8,BoilingtheSolutionwith30minutes,thenfilteringthesolutionandcarriedouttheresidueagain,CollectingthefiltrateinbeakerandAdjustingthefilter’sPHto5withconcentr

7、atedhydrochloricacid.Standingthefilterandprecipitatingcompletely.Collectingtheprecipitatebythefiltration.Theprecipitateiscrudeextractrutin.Recrystallizatingthecrudeextractrutinin60-70℃waterbathandboilingtherecrystallizate’ssolutiononelectricfurnacewith15minutes.Filtratingthesoluti

8、onwhenitishotandthefiltrateasideu