杀伤细胞免疫球蛋白样受体基因多态性与原发性胆汁性肝硬化的相关性分析.pdf

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1、细胞与分子免疫学杂志(ChinJCellMolImmuno1)2015，31(185·论著·文章编号：1007—8738(2015)01—0085—04杀伤细胞免疫球蛋白样受体基因多态性与原发性胆汁性肝硬化的相关性分析郑立明，许联红，何梅芳，史琼英，李欣，王永忠(常州市第三人民医院检验科，江苏常州213001；江苏大学附属武进人民医院检验科，江苏常州213002)[摘要]目的探讨江苏汉族人群杀伤细胞免疫球蛋白样受体(KIR)基因多态性与原发性胆汁性肝硬化(PBC)的相关性。方法收集2011—06—01／2013—07—31期间江苏省常州三院住院的P

2、BC患者122例和198例相匹配的无血缘关系健康人群外周血标本。采用实时荧光定量PCR检测KIR基因多态性，分析KIR基因及基因型频率在2组人群间的差异。结果PBC患者K／R2DL2和2DS2基因频率比对照组显著性降低，与降低疾病的患病风险有关。共检测出55种KIR基因型，其中PBC组24种，对照组47种。最常见的基因型为1，其次为BX2、BX8、BX4、BX14。对存在于5个或以上个体的11个基因型进行分析，未发现PBC组与对照组之间有显著性差异。结论K／R2DL2和2DS2的缺失可能是江苏地区汉族人群PBC发病潜在的保护因子。[关键词]杀伤细胞

3、免疫球蛋白样受体；基因多态性；基因型；原发性胆汁性肝硬化[中图分类号]Q343．15，R392．2，R593．2[文献标志码】ACorrelationanalysisbetweenMilercellimmunoglobulin-likereceptorgenepolymorphismandprimarybiliarycirrhosisZHENGLiming，XULianhong，HEMeifang，SHIQiongying，LIXin，WANGYongzhongDepartmentofClinicalLaboratory，ChangzhouThir

4、dPeople’sHospital，Changzhou213001；DepartmentofClinicalLaboratory，WujinPeople’SHospital，JiangsuUniversity，Changzhou213002，China【AbstractJObjectiveToexploretheassociationbetweenkillercellimmunoglobulin-likereceptor(KIR)genepolymorphismandprimarybiliarycirrhosis(PBC)indiangsuHanp

5、opulation．MethodsPeripheralbloodsamplesweretakenfrOm122patientswithPBChospitalizedinthirdpeople’shospitalofChangzhouinJiangsuprovincebetweenJune2011andJuly2013and198randomlymatchedunrelatedhealthydonors．KIRwastypedbySYBR。GreenIreal-timePCRgenotypingassay．Geneandgenotypefrequen

6、ciesofKIRwerecomparedbetweenthetwogroups．ResultsThegenefrequenciesofKIR2DL2and2Db"2weresignificantlylowerinthePBCgroupascomparedwiththoseofthecontrolgroup．KIR2DI_2and2DS2wereassociatedwithlowerriskofPBC．Thetotal55KIRgenotypeswerefoundinthepatients(24genotypes)andthecontrols(47

7、genotypes)．ThemostcommonlyobservedKIRgenotypewasAA1，andthenextwere脱，，BX4andBXI4．Only11genotypeswereobservedinfiveindividualsormore，allowingforameaningfulcomparisonofgenotypefrequenciesbetweenthepatientsandcontrols．Therewasnostatisticallysignificantdiferenceingenotypefrequencie

8、sbetweenthetwogroups．ConclusionTheabsenceofKIR2DI~／2DS2genoty