资源描述：
《cd及toll样受体基因多态性与大肠癌的相关性研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、CD14及Toll样受体4基因多态性与大肠癌的相关性研究中华消化杂志2006年11旦第鲞笙g:!rIl!!?(!(!?(CD14及Toll样受体4基因多态性与大肠癌的相关性研究陈丽珊郭秋莎夏冰【摘要】目的探讨我国湖北汉族人Toll样受体(TIR)4基因Asp299Gly和CD14C一260T基因多态性分布与大肠癌的相关性.方法采用聚合酶链反应限制性片段长度多态性(PCR—RFIP)方法,检测11o例大肠癌患者及160例正常对照者TIR4基因Asp299Gly及CD14c一260T基因型及等位基因频率的分布.结果大肠癌组CD14C一260
2、T基因型与正常对照组比较,差异有统计学意义(P<0.05).正常对照组CC基因型的频率为15.6,明显低于大肠癌组的31.8(P一0.0027,OR一0.3968,95CI一0.2209t0.7129);正常对照组中CT基因型的频率为48.1%,明显高于大肠癌组的30.9(P一0.0056,OR一2.074,95CI一1.246～3.452).所有样本中均未发现TIR4基因Asp299Gly的突变型.结论CD14C一260T基因多态性与中国湖北汉族大肠癌显着相关,而TIR4基因Asp299Gly多态性与大肠癌无关.【关键词】大肠癌;
3、基因多态性;CD14;Toll样受体4PolymorphismofCDI4genepromoterbutnotToll-likereceptor4geneisassociatedwithcolorectalcancerinChinesepatientsCHENLi—shan,GUOQiusha,XIABing.DepartmentofInternalMedicine&Geriatrics.ResearchCenterofDigestiveDiseases,ZhongnanHospital,WuhanUniversity,Wuhan
4、430071,ChinaCDrresp0gauthorXIABing,Email:bingxia2004@yahoo.tom.cn[Abstract]ObjectiveToinvestigateanassociationbetweenTolllikereceptor(TLR)4Asp299GlyorCD14C260TpolymorphismsandcolorectalcancerinChinesepatients.MethodsByamethodofpolymerasechainreactionbasedrestrictionfragme
5、ntlengthpolymorphism(PCRRFIP),TLR4Asp299GlyandCD14C260Tpolymorphismsinunrelated110patientswithcoloreetalcancerand16OhealthycontrolsinChineseHanracepopulationweregenotyped.ResultsSignificantdifferencesofCD14genotypeswerefoundbetweenhealthycontrolsandthepatientswithcolorect
6、alcancer.ThefrequencyoftheCCgenotypeinhealthycontrolswasextremelylowerthanthatinthegroupofcolorectalcancer(15.6vs.31.8,P一0.0027,OR一0.3968,95CI:0.22090.7129)whilethefrcquencyoftheCTgenotypeinhealthycontrolswassignificantlyhigherthanthatinthegroupofcolorectalcancer(48.1vs.3
7、0.9,P一0.0056,OR一2.074,95CI:1.2463.452).NoTIR4Asp299GlymutantgenotypewasdetectedinanypatientsandhealthycontrolsinChineseHanpopulation.ConclusionThepolymorphismofCD14C260TbutnotTLR4Asp299Glyisassociatedwithcolorecta1cancerinChinesepatients.[Keywords]Colorectalcancer;Genepol
8、ymorphism;CD14;Toll—likereceptor4大肠癌的病因,发病机制与遗传及环境等因素有关,但目前仅有少数基因被发现与大肠癌的遗传易感性相关.细菌脂多糖(LPS)是革兰阴性
