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1、Toll样受体4基因多态性与消化性溃疡和幽门螺杆菌感染的相关性研究?408?Toll样受体4基因多态性与消化ChinJGastroenterol,2006,Vo1.11,No.7性溃幽门螺杆菌感染的相关性研究疡和华开罗夏冰z李春z郭秋莎z夏华向武汉大学医学院附属中山医院消化内科'(430033)武汉大学中南医院综合医疗科武汉大学医学院消化系病研究中心过敏与免疫相关性疾病重点实验室2香港大学玛丽医院内科3背景:幽门螺杆菌(H.pylori)感染是消化性溃疡的主要病因.脂多糖(tI'S)通过T0U样受体(TLR)4激活核因子(NF).KB,在抗感染免疫应答中起启动和调节作用.TLR4基因发
2、生Asp299Gly突变可中断TLR4介导LPS信号传导.目的:研究我国湖北省汉族人群TLR4基因Asp299Gly多态性与消化性溃疡和pylori感染的关系.方法:采用病例对照研究和聚合酶链反应.限制性片段长度多态性(PCR.RFLP)法.检测126例消化性溃疡患者和264名正常对照者的TLR4等位基因Asp299Gly基因型分布.结果:消化性溃疡者pylori阳性率(90.5%)显着高于正常对照组(61.7%)(P<0.000l,OR=5.889,95%CI:3.089一l1.216).在H.pylori感染相关性消化性溃疡组和正常对照组中均未发现TLR4基因Asp299Gl
3、y的突变型,其基因型,等位基因以及携带者频率总体分布无显着性差异.结论:本研究未能显示TLR4基因Asp299Gly基因多态性与H.pylori感染,H.p',相关性消化性溃疡形成有相关性.关键词Toll样受体4;螺杆菌,幽门;脂多糖类;基因;多态性,单核苷酸AssociationbetweenToll-likeReceptor-4GenePolymorphismandHelicobacterpyloriInfectioninPepticUlcerDiseaseHUAKailuo,XIABing,LIChun,GUOQiusha,XIAHuaxiang.DepartmentofGast
4、roenterology,AffiliatedZhongshanHospitalofWuhanUniversitySchoolofMedicine,Wuhan(430033)Background:Helicobacterpylori(H.pylori)isamajorcauseofpepticulcer.Toll-likereceptor(TLR)4isamemberofnuclearfactor(NF)-KBactivatorsthatisknowntobindlipopolysaccharide(LPS)throughtheirLPRdomain.TheAsp299Glymutat
5、ioninterruptsTLR4-mediatedLPSsignaling.fl~llS"-TostudythedistributionofTLR4geneAsp299GlypolymorphismandtodefinetherelationshipbetweenTLR4genotypeandH.pylori-associatedpepticulcerinChinesepopulationwithHannationalityinHubeiprovince.Methods:Onehundredandtwentysixpatientswithpepticulcerand264health
6、ycontrolsweregenotypedbypolymerasechainreactionrestrictionfragmentlengthpolymorphism(PCR-RFLP)methodforTLR4geneAsp299Glypolymorphism.Results:H.pyloriinfectionwasdetectedin90.5%of126patientsand61.7%of264healthycontrolsf户l<0.0001,oddsratio=5.889,95%CI:3.089-11.216).Thedistributionsofthegenotype
7、andallelefrequenciesofTLR4Asp299Glywerenotsignificantlydifferentinthegroupsstudied.AllthesubjectstestedwereofTLR4genewildtypeA.Conclusions:Thenon-synonymoussinglenucleotidepolymorphismAsp299GlyinTLR4geneisrareandisnotassocia
