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《甲状旁腺激素对HAEC细胞EndMT诱导作用及机制探讨.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、垒生旦i箍墨鲞茎旦基目JBloodPurif,September,2014,Vo1.9,No.13·647··基础研究·甲状旁腺激素对HAEC细胞EndMT诱导作用及机制探讨李宁李亚斐【摘要】目的探讨甲状旁腺激素(parathyroidhormone,PTH)是否诱导血管内皮细胞上皮细胞一间充质转化(endothelial—mesenchymaltransition,EndMT)及相关机制。方法血管内皮细胞HAEC培养至对数期,分别加入不同剂量的全长重组PTH(10mol/L、10mol/L、10omol/L、10mol/L、10-smo1/L)作用48h;再
2、在10mol/LPTH作用下,选择12、24、36、60h4个时间点;分别收集总蛋白。Westernblot分析Ve—Cadherin、CD31、ct—SMA、TGF—B1和ILK的表达改变。结果Westernblot结果表明在PTH作用下,血管内皮细胞标志分子We—Cadherin和CD31表达降低;纤维细胞标志分子a—SMA表达则显著升高,且呈时间和剂量依赖性。Westernblot分析发现TGF—B1和ILK表达同时升高。结论PTH减弱内皮细胞特征、增强纤维化特征,诱导HAEC细胞发生EndMT,TGF-卜ILK通路是其调控的可能通路之一。【关键词】甲状
3、旁腺素;内皮细胞.间充质转化;尿毒症;整合素连接激酶中图分类号:R318.16文献标识码:Adoi:10.3969/j.issn.1671—4091.2014.09.0101[1leefectandmechanismofparathyroidhormoneinducedendothelia1.mesenchymaltransitionofvascularell-dotheliMHAECcellsLINing,LIl一feiInstituteofPreventiveMedicine,ThethirdMilitaryMedicalUni-versi~Chongqi
4、ng40003DepartmentofNephrology,刀FirstAfiliatedHospital,刀thirdMilitaryMedl"-calUniversi~Cbongqing400038.ChinaCorrespondingauthOF:LIl厂aEmail:liyafei2OO8@hotmail,corn[Abstract]ObjectiveToinvestigatewhethertheincreasedparathyroidhomaone(PTH)asauremictox.ininducestheendothelial—mesenchyma
5、ltransitionfEnd/vlT)ofvascularendothelialcellsfHACEcells)anditsrelatedmechanisms.MethodsThevascularendothelialHACEcellswereculturedtologarithmicphase.andPTHattheconcentrationof10-12,10一l1,10.10,l0.9,and10.8mol/Lwasaddedintothemediumfor48h.Additionally,HACEcellswereculturedinthemediu
6、mcontaining10.8mol/LPTHfor12,24,36.and60h.CellswerethenharvestedformorphologicalexaminationandforVECadherin,CD31,a.SMA.TGF一131andILKexpressiondeterminationsbywesternblot.ResuitsMorphologicalexaminationshowedthattheHACEcellstendedtohavefibrosischangesafterthePTHinduction.Wstemblotdis
7、playedthatPTHreducedtheex.pressionofvascularendothelialcellmarkersCD31andVECadherinandincreasedtheexpressionoffibercellmarkersa-SMAintimEanddosEdependentmanners.AtthesametimetheexpressionsofTGF.131andILKiB.creased.ConclusionPTHinducedtheHAECcellstohavelessendothelialcellcharacterist
8、icsandmorefi.brosis
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