复方守宫散对Lewis肺癌小鼠JAK2-STAT3信号通路的影响.pdf

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1、2014年5月第4卷第9期·论著·复方守宫散对Lewis肺癌小鼠JAK2-STAT3信号通路的影响1△2▲211沈迪李崇慧周晋华徐海惠周鹏飞1.安徽中医药大学，安徽合肥230000；2.安徽中医药大学第一附属医院肿瘤科，安徽合肥230000[摘要]目的观察复方守宫散对Lewis肺癌小鼠JAK2-STAT3信号通路的影响。方法将55只C57BL/6J小鼠按常规方法接种Lewis肺癌瘤株，6d后选取荷瘤成功的小鼠50只随机分为5组：阳性对照组、西药组、中药高剂量组、中药中剂量组、中药低剂量组，每组10只。阳性对照组：灌服生理盐水0.2mL，1次/d；中药组灌服复方守宫散高剂量组0.6mL

2、（相当于120mg/kg），中剂量组0.4mL（相当于60mg/kg），低剂量组0.2mL，（相当于40g/kg），1次/d；顺铂组按1mg/kg给药，0.1mL/只腹腔注射。每天1次，共14d。在光镜下用HE染色法观察肿瘤组织病理形态学改变情况，用WesternBlot法检测JAK2、STAT3蛋白的表达水平。结果各给药组JAK2、STAT3蛋白的表达水平出现不同程度降低，且JAK2降低较为明显。结论复方守宫散能够抑制肿瘤生长，降低Lewis瘤细胞中JAK2、STAT3蛋白的表达，并通过此方式阻断JAK2-STAT3信号转导。[关键词]肺癌；信号通路；实验研究[中图分类号]R734

3、.2[文献标识码]A[文章编号]2095-0616（2014）09-17-05StudyontheeffectsofCompoundShougongPowderontheJAK2-STATsignalingpathwayofmicewithLewislungcarcinoma12211SHENDiLIChonghuiZHOUJinhuaXUHaihuiZHOUPengfei1.AnhuiUniversityofChineseMedicine,Hefei230000,China;2.DepartmentofOncology,FirstAffiliatedHospitalofAnhuiU

4、niversityofChineseMedicine,Hefei230000,China[Abstract]ObjectiveToobservetheeffectsofCompoundShougongPowderontheJACK2-STAT3signalingpathwayofmicewithLewislungcarcinoma.Methods55C57BL/6JmicewereinoculatedwithtumorcelllinesofLewislungcarcinomaviaaconventionalmethod.50tumor-bearingmicewereselecteda

5、ftersixdaysandassignedtofivegroups:apositivecontrolgroup,awesternmedicinegroup,aTCMgroupwithhighdosages,aTCMgroupwithmediumdosages,andaTCMgroupwithlowdosages,with10miceineachgroup.Thepositivecontrolgroupwasorallygivennormalsalineof0.2mLonceaday;theTCMgroupswereorallygivencompoundshougongpowdero

6、f0.6mLforthegroupwithhighdosages(equalto120mg/kg),0.4mLforthegroupofmediumdosages(equalto60mg/kg),and0.2mLforthegroupwithlowdosages(equalto40mg/kg)onceaday;theWesternmedicinegroupwasintraperitoneallyinjectedwithcisplatinof1mg/kg,0.1mLeach,onceadayfor14days.Pathologicalandmorphologicalchangesoft

7、umortissueswereobservedviaHEstainingunderlightmicroscope,andexpressionlevelsofJAK2andSTAT3proteinsweretestedviatheWesternBlot.ResultsExpressionlevelsofJAK2andSTAT3proteinsineachgroupdecreasedwithdifferentdegrees,andthedecreaseofJA