顺铂对lewis肺癌小鼠体内mdsc的影响及其可能机制的探讨

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1、万方数据复旦大学硕士学位沦文复旦大学硕士学位论文顺铂对Lewis肺癌小鼠体内佃SC的影响及其可能机制的探讨Thestudyontheea℃ctandpossiblemechanismofcisplatinonMDSCsinT●1●‘LeWlSlungCanCerbearingmiCe院系：复旦大学附属华山医院专业：肿瘤学姓名：王甜指导老师：梁晓华主任医师导师小组：周鑫莉副主任医师黄若凡主治医师完成日期：2013年4月2日万方数据目录中英文缩略词表·····································中文摘要··················

2、··-·-····················英文摘要···········································引言···············································材料和方法·········································1．材料·····························-···一·一··1．1实验动物·······························1．2细胞来源······························

3、·1．3主要试剂···························-·1．4所需溶剂配制方法·······················1．5主要耗材和仪器··················-·-····2．实验方法与步骤·····························2．1细胞培养······························2．2Lewis肺癌模型鼠建立及给药············2．3标本采集········-····················2．4操作步骤····························结果

4、·········-····································1顺铂对小鼠体内如Sc的数量的影响··-·······1．1顺铂对硒sC在肿瘤组织中数量的影响····1．2顺铂对如SC在荷瘤小鼠脾脏中数量的影响··2．顺铂对小鼠体内Galectin_3的影响··········l258地挖拢抱他屹媳M坫垢加毖船万方数据2．1．Galectin一3在荷瘤小鼠组织中表达量的变化···232．2．Galectin一3在荷瘤小鼠外周血中表达量的变化··253．Galectin一3在Lewis肺癌细胞中的表达·········264．Galec

5、tin一3在如SC中的表达····················285．Galectin一3对肋SC的趋化作用·--············296．Galectin一3对如SC的凋亡的影响··············317．Galectin一3促进骨髓前体细胞分化为如SC·······32讨论····················-·--·-··-···················34结论·········································-······38创新点·······························

6、··············39参考文献···········································40综述····························-··-·-·············47致谢···············································58声明············-··································59万方数据复旦大学硕士学位论文中英文缩略词表Abbreviation万方数据复旦大学硕士学位论文顺铂对Lewis肺癌小鼠体内如SC的影

7、响及其可能机制的探讨中文摘要【目的】化疗药物在损伤肿瘤细胞的同时也干扰机体的免疫系统。化疗后荷瘤机体内的免疫格局形成对肿瘤的复发、进展等有重要意义。髓系来源抑制性细胞(MyeliodDerived—suppressorCells，MDSCs)是诱导机体产生免疫抑制的重要细胞，并成为肿瘤诱导的免疫耐受的研究热点之一。MDSC作为一群未成熟的免疫抑制性细胞在包括肺癌在内的多种肿瘤机体中异常聚集，这与体内的诸多调节性因子发生变化有关。活细胞损伤释放出具有免疫调节活性的损伤相关分子模式(DamageAssociatedMolecularpatterns，DAMPs)，半

8、乳糖凝集素3(Galec