含缬酪肽蛋白基因单核苷酸多态性在肝细胞癌中的研究-论文.pdf

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1、·238·JSurgConceptsPract2014，Vo1．19，No．3．论著．含缬酪肽蛋白基因单核苷酸多态性在肝细胞癌中的研究刘学谦，王新景，张天，邓侠兴，陈皓，沈柏用，彭承宏(上海交通大学医学院附属瑞金医院外科，上海200025)【摘要】目的：探讨含缬酪肽蛋白(valosincontainingprotein，VCP)基因不同位点单核苷酸多态性(sin异lenucleotidepolymorphism，SNP)与肝细胞癌(hepatocellularcarcinoma，HCC)的关系。方法：采用病例组与对照组研究，收集122例HCC(HCC组)和120例非HCC(对照组)

2、外周血标本，对VCP基因的4个标签SNPs采用直接测序法进行基因测定分型采用卡方检验比较基因型及等位基因在HCC组与对照组之间分布的差异，采用非条件Logistic回归分析多态基因型与HCC的关系。结果：rs546982位点等位基因及基因型在HCC组与对照组中的分布具有显著性差异(P<0．05)携带rs546982位点AA基因型者比GG基因型者发生HCC风险低，而携带AA基因型的HCC病人比携带GG基因型的HCC病人发生脉管癌栓及淋巴结转移的风险高。位点rs2074549、rs607671、rs10972300的基因型在HCC组和对照组之间差异无统计学意义结论：VCP基因多态性与H

3、CC存在关联．rs546982位点AA基因型可降低HCC发生率．但可增加HCC病人的淋巴结转移及脉管癌栓的风险。关键词：含缬酪肽蛋白：单核苷酸多态性：肝细胞癌中图分类号：R735．7文献识别码：A文章编号：1007—9610(2014)03．0238．05DOI：10．39696．issn．1007—9610．2014．03．013SinglenueleotidepolymorphismsofvalosincontainingproteingeneinhepatoceHularcarcinomaLIUXueqian，WANGXinjing,ZHANGTian,DENGXiaxing

4、,CHENHao，SHENBaiyong,PENGChenghong．DepartmentofSurgery,Ru~inHospital,ShanghaiJiaotongUniversitySchoolofMedicine，Shanghai200025,China【Abstract]ObjectiveToexploretheassociationbetweensinglenucleotidepolymorphism(SNP)ofvalosincontainingprotein(vcP1andhepatocellularcarcinoma(HCC)．MethodsInthiscase

5、—controlstudy，fourtag—SNPsofVCPgenewereanalyzedbydirectgenotypingofDNAfromperipheralbloodspecimenscollectedfrom122caseswithHCC(HCCgroup)and120non—HCCeases(controlgroup)．Chi—squaretestwasperformedtocomparethediferencesofdistributionofgeno—typesandallelesbetweengroups．Theassociationbetweenpolymo

6、rphicgenotypeandHCCwasanalyzedbytheuncondi—tionedLogisticregression．ResultsThedistributionofbothallelesandgenotypesofrs546982differedsignificantly(P<0．051betweentheHCCgroupandthecontrolgroup．IndividualscarryingAAgenotypeof~546982hadalowerriskofHCCthanindividualscarryingGGgenotype．However，HCCpa

7、tientscarryingAAgenotypeofrs546982enduredahigherriskoflymphnodemetastasisandvasculartumorembolism．NodiferenceexistedbetweentheHCCgroupandthecontrolgroupfor~2074549，m607671andm10972300polymorphisms．ConclusionsTheresultssuggestedthatVCPpo