BMSC-肝细胞共培养上清治疗大鼠急性肝衰竭疗效观察-论文.pdf

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1、第11卷第3期Jou延rna安lo大fY学an学an报U(n医iv学ers科ity学(版Me)dSci)Vo1．11No．32013年9月Sep．2013BMSC一肝细胞共培养上清治疗大鼠急性肝衰竭疗效观察霍大浪，宁家辉，胡文君，刘明颖2，张袁媛，王娜’，冯成军，王英杰2(1．遵义市第一人民医院感染病科，贵州遵义563000；2．第三军医大学西南医院感染病研究所，重庆400038)摘要：目的探讨骨髓间充质干细胞(bonemarrowmesenchymalstemcells，BMSC)一肝细胞共培养上清治疗大鼠急性

2、肝衰竭(acuteliverfailure，ALF)的疗效，为其临床应用提供实验依据。方法分别利用肝细胞、BMSC、BMSC一肝细胞培养上清治疗腹腔注射D一氨基半乳糖胺(D—ga1)诱导的大鼠ALF模型，观察各治疗组动物的l周存活率，肝功能和肝组织病理改变。结果肝细胞、BMSC及两者共培养上清治疗组大鼠存活率显著高于对照组(P<0．01)，其中共培养上清治疗组大鼠存活率最高达80％；各治疗组ALT、AST、TBIL较治疗前均显著降低(P<0．O1)，共培养上清治疗组下降最明显；各治疗组肝脏病理组织学损害明显减轻，

3、对照组损害加重。培论BMSC一肝细胞共培养上清能更有效的救治大鼠ALF模型，有望为ALF的治疗提供了新的治疗途径。关键词：急性肝衰竭；细胞共培养；骨髓间充质干细胞；肝细胞；上清治疗中图分类号：11575．3文献标识码：A文章编号：1672—2639(2013)03—0008—04ThetherapeuticpotentialofsupernatantderivedfromCO—cultureofBMSCandhepatocytesinthetreatmentofacuteliverfailureHUODa—lan

4、g，NINGJiQ—hui’，HUWen-janz，UUMing—ying。ZHA{、4GYuan—yuan，WANGN，FENGCheng-jun，WANGYing-jie。(1．Departmentofinfectiousdiseasesofthefirstpeople~HospitalofZunyiCity，GuiZhou563000，China；2．InstitutionofInfectiousDiseases，SouthwestHospitalofThirdMilitaryMedicalUniversi

5、ty，Chongqing400038，China)Abstract：ObjectiveToinvestigatethetherapeuticpotentialofsupernatant，whichisderivedfromCO—cuhureofbonemarowmesenchymalstemcells(BMSC)andhepatocytes，inthetreatmentofacuteliverfailure(ALF)rats．MethodsSupernatantsderivedfromhepatocytes，BM

6、SCorco—cultureofbothwereintraperitonealinjectedintoD—galactosamineamine(D—ga1)inducedratswiththeALFphenotype．Survivalrate，liverfunctionsandhistopa—thologywereexaminedbeforeoraftertransplantation．ResultsSupernatantsfromdifferentcellscanimprovesurvivalrate，live

7、rfunctionsandhistology．SupernatantderivedfromCO—cultureofBMSCandhepatocyteswasfoundtobemoreeficientthansupernatantsderivedfromBMSCorhepatocytesinthetreatmentofALF(P<0．01)．Conclu-sionOurfindingsuggestthatsupematantinjectionofCO——cultureofBMSCandhepatocytesisan

8、ovelapproachfortreatingacuteliverfailureandmaybeapromisingtherapeuticinterventioninthefuture．Keywords：acuteliverfailure(ALF)；cellCO—culture；bonemarrowmesenchymalstemcells(BMSCs)；livercell