小白菊内酯诱导肝癌细胞SMMC 7721自噬性死亡的实验研究-论文.pdf

《小白菊内酯诱导肝癌细胞SMMC 7721自噬性死亡的实验研究-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、四川大学学‘医学版JSichuanUniv(MedSciEdi)2～。l4一；4。5。(4)⋯：58。7—5。9。dE小白菊内酯诱导肝癌细胞SMMC7721自噬性死亡的实验研究刘战培，李艳艳，高波，黎军，高俊萍，林苹。△1．中航工业三六三医院普外科(成都610041)；2．四川I大学华西医院实验肿瘤研究室(成都610041)【摘要】目的探讨小白菊内酯对肝癌细胞SMMC7721自噬反应的影响及其机制。方法通过MTT测定小白菊内酯对SMMC7721细胞活力的影响；通过吖啶橙染色观察不同浓度小白菊内酯对SMMC7721细胞自噬的影响，Wester

2、nblot检测细胞自噬标志物LC3、p62的表达以及自噬流检测分析小白菊内酯对细胞自噬的影响；用抗氧化剂NAC去除细胞中ROS，通过Westernblot检测小白菊内酯对细胞自噬的影响，确定ROS在其中的作用。结果小白菊内酯可抑制SMMC7721细胞的增殖，并具有浓度依赖性；通过白噬的标志物及自噬流的检测显示小自菊内酯可诱导肝癌细胞系SMMC7721细胞发生自噬，用NAC去除ROS后，小白菊内酯引起的自噬明显下降，说明其诱导的SMMC7721细胞发生自噬依赖于R()s。结论小白菊内酯可能是通过刺激细胞产生R()s诱导sMMC7721肝癌细胞

3、发生自噬性死亡。【关键词】小白菊内酯肝细胞癌自噬活性氧FeverfewLactoneInducesAntophagicDeathofHepatocellularCarcinomaSMMC7721CellsLIUZhan—pei，LIYan—yan。，GAOBo，L1Jun，GAOJan—ping，LJNPing．1．DepartmentofGeneralSurgery，AVIC363Hospital，Chengdu610041，China；2．DivisionofExperimentalOneology，StateKeyLaboratory

4、ofBiotherapy，WestChinaHospital，SichuanUniversity，Chengdu610041，China△Corresp0ndingauthor，E—mail：linping8@yahoo．corn[Abstract]ObjectiveToexploretheeffectandmechanismoffeverfewlactoneoninducingautophagicdeathofhepatocellularcarcinoma．MethodsTheproliferationofhepatocellularca

5、rcinomaSMMC7721celtStreatedwithfeverfewlactonewasmeasuredbyMTTassay．TheautophagyofSMMC7721inducedwithfeverfewlactonewasassessedbyacridineorangestaining，autophagicmarkerLC3andp62detectingandautophagicflowsanalyzing．Inaddition．aroleofROSinthisprocesswasstatedbytreatmentwitha

6、ntioxidantagentN～acetyl—I一cysteine(NAC)．ResultsTheproliferationofSMMC7721cellswereinhibitedbyfeverfew1actoneinaconcentrationdependencemanner．TheexpressionofLC3andautophagicflowsofSMMC7721cellswereincreasedbyfeverfewlactone，whilep62wasdecreased，whichimpliedthatfeverfewlacto

7、necouldinducetheautophagyofSMMC7721cells．Furthermore，theautophagyeffectinducedbyfeverfewlactonewasdeclinedobviouslywhentraetedwithNACsuggestedthatROSplayedanimportantroleinthiseffect．ConclusionFeverfewtactoneinducesautophagicdeathofSMMC7721cellsbystimulatingcellstOproduceR

8、OS．ThestudywillbehelpfulforthetreatmentofhepatocellularcarcinomaandtOprovidetheoreticalba