全反式维甲酸对人肺腺癌A549体外增殖的影响及其机制探讨-论文.pdf

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1、重庆医学2014年3月第43卷第7期8O5论著·临床研究全反式维甲酸对人肺腺癌A549体外增殖的影响及其机制探讨梁宇佳，杨小琼，兰四友，秦艳。，邓述恺(1．泸州医学院附属医院呼吸内一科，四川泸州646000；2．成都龙泉驿区医院呼吸内科，成都610100)摘要：目的观察全反式维甲酸(ATRA)对人肺腺癌细胞A549增殖的影响，并对其机制进行初步的探讨。方法以肺腺癌A549细胞株为研究对象，用噻唑蓝(MTT)法检测试验组和对照组细胞，计算细胞的生长抑制率；用透射电镜观察细胞细微结构变化情况；用酶联免疫吸附试验测定细胞上清液中环氧化酶一2(COX一2)的水平变化。结果不同试验

2、浓度的ATRA处理肺腺癌A549细胞，均能对A549细胞产生不同程度的抑制作用，且经过72h后，各试验浓度组均可见C0X一2在细胞质中的表达明显下降，并与浓度呈正相关，差异有统计学意义(P

3、全反式维甲酸doi：10．3969／．．issn．1671-8348．2014．07．013文献标识码：A文章编号：167l一8348(2014)07-0805-04InfluenceofATRAonin。vitroproliferationofhumanlungadenocarcinomacelllineA549anditsmechanismsLiangYujia，YangXiaoqiong，LanSiyou，QinYan，DengShukai(1．FirstDepartmentofRespiration，AffiliatedHospitalofLuzhouMedica

4、lCollege，Luzhou，Sichuan646000，China；2．DepartmentofRespiration，LongquanyiDistrictPeople'sHospital，Chengdu，Sichuan610100，China)Abstract：ObjectiveToexploretheinfluenceofall—transretinoicacid(ATRA)onin—vitroproliferationofhumanlungadeno—carcinomacelllineA549andtopreliminarilystudyitsmechanism

5、．MethodsThehumanlungadenocarcinomacelllineA549wastakenastheexperimentalgroup．Thecellsintheexperimentalgroupandthecontrolgroupweredetectedbyusing3一(4，5-dimethyl—thiazol一2一y1)一2，5-diphenyltetrazoliumbromide(MTT)assayforcalculatingthecellgrowthinhibitionrate；themicrostructureofthecuhuredcell

6、swasobservedbythetransmissionelectronmicroscopetechnique；theexpressionofcyclooxygenase一2(COX-2)inthece11supernatewasdetectedbytheenzymelinkedimmunosorbentassay．ResultsThedifferentconcentrationsofATRAfortreatingA549ce11scouldproducetheinhibitingeffectonA549cellstosomedifferentdegrees，furth

7、ermore，after72h，theexpressionofCOX一2incytoplasmaamongthevariousconcentrationsgroupswassignificantlydecreased，whichwaspositivelycorrelatedwiththeconeentration。thedifferenceshowingstatisticalsignificance(P<0．05)；atthesametime，bydetectingTRAILexpressionlevelintheexperi