地塞米松联合顺铂对肺腺癌A549细胞增殖和凋亡的影响-论文.pdf

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1、·762·余宗艳，等地塞米松联合顺铂对肺腺癌A549细胞增殖和凋亡的影响地塞米松联合顺铂对肺腺癌A549细胞增殖和凋亡的影响余宗艳，齐宇红，梁军Efectsofdexamethasonecombinedwithcisplatinofproliferationandapoptosisofhu-manpulmonaryadenOCarCin0macellK549YuZongyan，QiYuhong，LiangJunDepartmentofRadiotherapy，TangduHospitalofthe4thMilitaryMedicalUniversity，ShaanxiXi'an7

2、10038，China．【Abstract】Objective：Toinvestigatetheeffectsofproliferationandapoptosisandthemechanisminducedbydexa-methasone(DEX)andcisplatin(DDP)inhumanpulmonaryadenoearcinomaceUA549andpossiblemechanism．Methods：SubculturehumanpulmonaryadenocarcinomacellA549invitro，andwastreatedwithDEXandDDP．Thep

3、roliferationabilityoftheA549ceilsweredetectedbyMTrmethod．Thecellcycleandcellapoptosiswereanalyzedbyfloweytometry．TheexpressionlevelofvitaminDreceptorwasevaluatedbyWesternblot．Results：Theconcentra-tionofDEXabove500nmo~LcouldinhibittheproliferationofA549cellsobviously，theinhibitoryratedependson

4、theconcentrationandtime(P<0．05)．TheconcentrationofDEXlessthan500nmol／Lcouldntinhibittheprolifera—tionofA549cells(P>0．05)．TheconcentrationofDDPin0．3125—10p,g／mlcouldinhibittheproliferationofA549cellsobviously，theinhibitoryratedependsOiltheconcentrationandtime(P<0．05)．Thecellcycleandcellapopto—

5、sisanalyzedbyflowcytometry，theDDP+DEXgroupG1phaseandSphasecellpercentagedropstorisemoreobvious(P<0．05)．WesternblotobservedtheVDRexpressionlevelofDDP+DEXgroupincreasedsignificantly(P<0．05)．Conclusion：DEXcombinedwithDDPcouldincreacetheexpressionofVDRontheproliferationofA549cells，andpromottheapo

6、ptosisandinducecellcyclearrest，andimprovethecisplatinchemotherapysensitivity．【Keywords】dexamethasone．humanpulmonaryadenocarcinomacellA549；cisplatin；cellcycle；chemotherapysensi—tivityModemOncology2014，22(04)：0762—0766【摘要】目的：探讨地塞米松(dexamethasone，DEX)联合顺铂(cisplatin，DDP)在体外对肺腺癌A549细胞增殖和凋亡的影响及可能的机

7、制。方法：体外培养人肺腺癌A549细胞。MTF法检测DDP和DEX对A549细胞增殖的影响。流式细胞术分析细胞周期及细胞凋亡。Westernblot检测DEX和DDP对A549细胞内VDR表达水平的影响。结果：地塞米松>~500nmol／L浓度时对A549细胞有一定的增殖抑制作用(P<0．05)；<500nmoVL浓度时对A549细胞无明显抑制作用(P>0．05)；DDP浓度在0．3125一lOng／ml范围内对A549细胞增殖有明显的抑制作用，并呈浓度及时间依赖性(P<0．05