β-榄香烯联合顺铂对乳腺癌 MCF -7细胞增殖和凋亡的影响-论文.pdf

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1、匡堂生月第22卷第5期MODERNONCOLOGY．Mav．2014．VOL．22．NO．05·1005·l3一榄香烯联合顺铂对乳腺癌MCF一7细胞增殖和凋亡的影响郑瑾，马力天，任秦有，杨建栋，刘毅，张毅，范玉贞，张伟，张哲伟，瞿湘梅，杨明会Efectofp—ElemenecombinedwithDDPoftheproliferationandapoptosisofMCF——7celIZhengJin，MaLitian，RenQinyu，YangJiandong，LiuYi，ZhangYi，FanYuzhen，Zhang

2、Wei，ZhangZhewei，QuXiangmei，YangMinghuiDepartmentofTraditionalChineseMedicine，ChinesePGeneralHospital，Beijing100853，China；DepartmentIntegratedTraditionalandWesternMedicineofOncology，TangduHospital，eFourthMilitaryMedicalUniversity，ShaanxiXihn710038，China；StudentTea

3、ms，TheFourthMilitaryMedicalUniversity，Shaanxian710032，China；465547MilitaryHospital，LiaoningHaicheng114200，China．【Abstract】Objective：ToinvestigatetheefectofB—ElemenecombinedwithDDPontheproliferationandapop．tosisofMCF一7cel1．Methods：DifferentconcentrationsofB—Elemen

4、einjection(25，50，100，150，2001zg／m1)wereappliedtoMCF一7cells．CellproliferationwasmeasuredbyMTr．Cellapoptosisandcell—cyclewasdetectedbyflowcytometry．Theappropriatedrugconcentration(3tLg／mlDDP，125tLg／mlB—Elemene)wereselected．Then8一E1-emenecombinedwithDDPinectionwasap

5、pliedtoMTrcells．CellproliferationwasmeasuredbyMTF．Cellapoptosisandcell—cyclewasdetectedbyfloweytometry．Results：CellproliferationwasdramaticallyinhibitedbyB—Elemeneinjection(25，50，100，150，2001xg／m1)at24h，48h(P<0．05)．Inaddition，cellproliferationwasdramaticallyinhib

6、—itedbyinjectionB—ElemenecombinedwithDDP．Combinationtherapy，apoptosisrateandinhibitionrateofprolifera—tioninMCF一7cellstreatedbyp—E1emenecombinedwithDDPwashigherthanthemonotherapy(P<0．01)．Cell—cyclewasarrestedinGo／G1phasebyelemenecomparedtouninfeetiouscellsinthesa

7、metimescale．Conclusion：p—ElemeneandDDPcombinedwithp—Elemenecaninhibittheproliferation，acceleratetheapoptosisinMCF一7cells．DDPcombinedwithB—ElemenehavesynergisticeffectontheapoptosisinMCF一7cells．【Keywords】B—Elemene；DDP；MCF一7cell；proliferation；apoptosis；cell—cycleMo

8、dernOncology2014．22(05)：1005—1009【摘要】目的：探讨B一榄香烯联合顺铂对乳腺癌MCF一7细胞生长的影响。方法：体外培养乳腺癌MCF一7细胞，将B一榄香烯(浓度梯度为25、50、100、150、2001xg／m1)，单独作用于乳腺癌MCF一7细胞，加药24h、48h后用噻唑蓝(MYr法)