PS1转基因小鼠学习记忆能力的影响-论文.pdf

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1、解剖科学进展ProgressofAnatomicalSciences2014Jul，20(4)：344～348外源性NGF对APP／PS1转基因小鼠学习记忆能力的影响杨畅，刘玉丽。，李宁(辽宁中医药大学1．解剖组胚教研室；2．针灸推拿学院；3．重大科研平台，辽宁沈阳110847)【摘要】目的观察外源性神经生长因子(nervegrowthfactor，NGF)对APP／PS1转基因小鼠学习记忆能力的影响和环磷酸腺苷反应元件结合蛋白(cAMPresponseelementblingprotein，CREB)蛋白的调节作用。方法实验分为野

2、生组(雄性5月龄c57BL／6小鼠6只，鼻腔给予生理盐水)，AD组(雄性5月龄APP，Ps1小鼠6只)和AD+NGF组(雄性5月龄APP／PSld~鼠6只，鼻腔给予小鼠NGF治疗14w)，应用Morris水迷宫检测小鼠的学习记忆能力变化，应用Westernblot检测小鼠脑内CREB蛋白、p-CREB蛋白的表达。结果AD+NGF组小鼠的潜伏期和找到平台前所经过的总路程均显著低于AD组小鼠，经过目标平台位置的次数明显高于AD组小鼠，游泳总路程和平均游泳速度均明显低于AD组小鼠。外源性NGF上调APP／PS1转基因小鼠脑内CREB蛋白和

3、P—CREB蛋白的表达。结论外源性NGF改善APP／PS1转基因小鼠的学习记忆能力可能与上调CREB和P—CREB蛋白的表达相关。【关键词】神经生长因子；阿尔茨海默病；APP／PS1转基因小鼠；CREB【中图分类号】R749．1’6【文献标志码】A【文章编号】1006—2947(2014)04—0344—05EfectsofexogenousNGFonlearningandmemoryabilitiesoftheAPP／PS1transgenicmiceYANGChang，LIUYu—li，LINing(1．Departmentof

4、AnatomyandHistology＆Emb~ology，and2．DepartmentofAcupunctureandMass~eCollege，and3．MajorScientificResearchPlatform，LiaoningUniversityofTraditionalChineseMedicine，Shenyang110847，China)【Abstract】0bjectiveTostudytheeffectsofexogenousNGFonlearningandmemo~abilitiesandtheexpres

5、sionofCREBproteinintheAPP／PS1transgenicmice．MethodsThemiceweredividedinto：wildgroup，APP／PS1transgenicgroupandAPP／PS1+NGFgroup(treatedwithNGFvianasalcavityfor14weeks)．Morriswatermazetestwssappliedtoexaminethechangesoflearningandmemo~ofthemice．Westernblottechniquewasused

6、totesttheexpressionsofCREBproteinsandp-CREBproteinsinthemicebrain．ResultsTheescapelatencyandthetotaldistanceoffindingplatforms，thetotalswimmingdistancesandtheaverageswimmingspeedswerenotablylowerinAD+NGFgroupthaninADmice，butthetimesofpassingtargetplatformlocationwereob

7、viouslyhigherinAD+NGFgroupthaninADmice．TheexogenousNGFupregulatedtheexpressionsofCREBproteinsandp-CREBproteinsinbrainofAPP／PS1transgenicmice．ConclusionTheimprovementoflearningandmemo~abilitiesoftheAPP／PS1transgenicmicebyexogenousNGFmightberelatedtoupregulationofCREBand

8、p-CREBproteinexpression．【Keywords】nervegrowthfactor；Alzheimer’sdisease；APP~S1transgenicmice；cAMPresponseelementblingp