大枣对酒精性肝病小鼠肝组织CYP2E1和TNF-α表达的影响-论文.pdf

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1、中国中西医结合杂志2014年4月第34卷第4期CJrrwM，April2014，Vo1．34，No．4大枣对酒精性肝病小鼠肝组织CYP2E1和TNF—OL表达的影响申军华’李芳芳摘要目的观察大枣预处理对酒精性肝病小鼠血清AST、ALT，肝组织病理及肝组织细胞色素P4502e1(cytochromeP4502e1，CYP2E1)和肿瘤坏死因子0【(tumornecrosisfactor—or．，TNF·仅)表达的影响。方法88只昆明种小鼠随机分成空白对照组(简称对照组，28只)、模型组(32只)和大枣治疗组(简称治疗组，28只)，模型组和治疗组采用酒精灌胃的

2、方法复制动物模型，对照组应用蒸馏水代替。4周时治疗组灌胃大枣提取物，其余组均灌胃等量蒸馏水。共计12周。观察应用大枣后，酒精性肝病小鼠血清AST、ALT，肝组织病理及肝组织CYP2E1和TNF—or．的表达。结果与模型组比较，治疗组血清AST、ALT水平下降，肝组织病理改善，肝组织CYP2E1和TNF-or．表达下降，差异有统计学意义(P<0．05)。结论大枣对酒精性肝病有一定疗效。关键词大枣；酒精性肝病；细胞色素P4502E1；肿瘤坏死因子仅EfectofJuIubeontheExpressionofCYP2ElandTNF-aintheHepaticT

3、issueofMlcewithAlco-hollcLiverDiseaseSHENJUR-huaandLIFang-fang1DepartmentofGastroentero／ogy．FirstHospitalofHandanCity，Hebei(056001)，China；2DepartmentofBiochemistryandMolecularBiology，HebeiMedicalUniversity，KeyMedicalBiotechnologyLaboratoryofHebeiProvince，Shijiazhuang(050017，ChinaA

4、BS1RACTObjectiveToobservethee仟ectofIujubepretreatmentonserumlevelsofASTandALT，liverpathology，andtheexpressionofcytochromeP4502e1(CYP2E1)andtumornecrosisfactor—Or,(TNF—d)inthelivertissueofalcoholicIiverdisease(ALD)mice．MethodsTotally88Kunmingmicewererandomlydividedintothecontrolgro

5、up(n=28)，themodelgroup(n=32)，andthejujubetreatmentgroup(n=28)．Theanimalmodelwaspreparedusingintragastricalcoholismformiceinthemodelgroupandthejujubetreatmentgroup，whiledistilledwaterwasintragastricallygiventothoseinthecontrolgroup．ExtractionofjujubewasintragastricalIygiventomicein

6、thejujubetreatmentgroupatweek4，whileequalvolumeofdistilledwaterwasintragastricallygiventomiceintheresttwogroups．Thethera—peuticcourselastedfor12weeks．SerumlevelsofASTandALT，liverpathology，andtheexpressionofCYP2E1andTNF-intheIivertissueofALDmicewereobservedafteradministrationofiuju

7、be．ResultsComparedwiththemodelgroup，serumlevelsofASTandALTdecreased，theliverpathologywasim—proved，andtheexpressionofCYP2E1andTNF-Or．intheIivertissuedecreased．showingstatisticaldi仟er—ence(P<0．05)．ConclusionJujubehadcertaineffectintreatingALD．KEYWORDSiujube；alcoholicliverdisease；cyt

8、ochromeP4502e1；tumornecrosisfacto