Lupeol及其衍生／修饰物H2抗肝细胞肝癌作用及其机制的初步研究-论文.pdf

《Lupeol及其衍生／修饰物H2抗肝细胞肝癌作用及其机制的初步研究-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、282刘芬，等Luoeol及其衙生／修饰物12抗肝细胞肝癌作用及其机制的初步研究16]JiangK，HeB，LaiL，etalCyclosporineAinhibimbreastca⋯celgrowthbydownregulatingtheexpressionofpyruvatekinasesubtypeM2[J]lntJMolNed，2012，30(2)：302308"]lqbalMA，BamezaiRNResveranolinhibitscancercellmetabolismbydownregulatingpymvatekinaseM2viainhibitionof⋯malia

2、ntmgetofrapamycin[J]PLc~SOne，2012，7(5)：e3676418]ChenJ，XieJ，JiangZ，etalShikoninanditsanalogsinhibitcancercelglyeolysisbytargetingm⋯pyruvatekinaseM2[J]On一≤委娶洲cogene20^30(42)：42924306(编校：徐萌)⋯量_～恤～_耋～Lupe垂ol及m其衍一生／修饰m物一卜H2抗肝细胞肝癌作用及其机制的初步研究刘芬，何艳，李云森，朱永明。，刘海燕Anti—hepatocellularcarcinomaefectoflupeola

3、ndilsmodifiedcompoundH2andthemechanismsLiuFen，HeYan，LiYunsen，ZhuYongming，LiuHaiyanOncologyInstitute，TheFourthPeople~Ho~ital。}Wuxi，diangsuWuxi214062，China

4、_Laboratory。}Celularand酣0kc越Tumor1mmwu)logy，InstituteofBiologyandMedicalSciences，SoochawUniversity，．1ia~suSuzhou215123，China；SchoolofPharmacy

5、，MedicalCollegeSoochOWUniversity．JiangsuS^0M215123．Cina．【Abstract】Objective：Toinvestigatetheanti—hepatocellulareareinonlaefectoflupeolanditsmodifiedeol—pound，H2，andthelneehanislnsMethods：ThestructureoflupeolwasmodifiedMqTassaymodifiedcompound，142Flowcytomett7analysiswasusedtodetecttheapoptosisi

6、nducedbylupeol／H2Westernblotanalysiswasusedtode—tecttheexpressionlevelofcleaved—PARPproteinResults：MTTresultdemonstratedthatH2greatlyimprovedtheanti—hepatoceHulareareinonlaefectoflupeol㈣ICoflupeolwere45bunol／L(forSMMC7721)and485bunol／L(forHepG2)，whileIC5。ofH2WSS30bunol／L(f0rbothSMMC7721andHepG2

7、)Flowcytomett7resultshowedthat20／xlnol／LH2inducedapoptosisofSMMC7721cels，andWesternblotresultsindicatedthat20／xlnol／LH2increasedtheexpressionlevelofcleaved—PARPinSMMC7721eeHs．whereas20／xlnol／LlupeolhadlitlefunctionConclu-sion：Modifiedcompoundoflupeol，H2，significantlyimprovedanti—hepatocellulare

8、areinonlaefect【Keywords】hepatocdlularcarcinolna；lupeol；H2ModernOncology2f)14，22(02)：0282—0285【摘要】目的：探讨lupeol及其结构改造后衍生／修饰物H2抗肝细胞肝癌作用及机制。方法：对lupeol进行化学结构修饰，然后应用MqT方法检测lupeol及其衍生／修饰物H2对HepG2和SMMC7721细胞增殖的作用，流式细胞术检测lupeol、H2对SMMC7721细