T位点基因多态性与糖尿病肾病的相关性.pdf

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1、实用医学杂志2014年第3O卷第16期2547整合素et2(ITGA2)一807C／T位点基因多态性与糖尿病肾病的相关性廖雪玲陈军宁李小励邓特邓一岚谢旭摘要目的：探讨桂北地区汉族人群整合素or2(ITGA2)基因807C／T位点基因多态性与糖尿病肾病(DN)的关系及其对血脂、载脂蛋白水平的影响。方法：选择住院确诊的2型糖尿病患者206例，再根据DN的诊断标准，分为糖尿病非肾病组(T2DM)及DN组，另选90例健康体检者为对照组(NC)。运用常规检测方法检测三组实验组的血清血脂及载脂蛋白水平，应用聚合酶链反应一限制性片段长度多态性(PCR．RFLP)技术检测三组的基因型及等位基因频率分

2、布。结果：与对照组相比。DN组的血脂Tc、TG、LDL．C值均升高(P<0．05)，DN组ITGA2基因．807C／T基因型分布及等位基因频率均有统计学差异(P<0．05)；CT+Tr基因型及T等位基因频率显著高于对照组(均P<0．05)；携带T等位基因的个体患DN的相对风险是携带c等位基因的1．72倍(OR=1．72。95％C／=1．176～2．517)。而，I’2DM组与对照组、DN组相比，ITGA2基因．807C／T基因型分布及等位基因频率差异均无统计学意义f均P>0．05)。结论：ITGA2．807C／T基因多态性与广西桂北地区汉族人群的DN发病具有一定的相关性。其中T等位基

3、因可能是DN发病的遗传易感基因，T等住基因可促进血脂TC水平的高度表达而使DN的发病风险增加。关键词糖尿病肾病；整合素2：基因多态性AcorrelationresearchbetweentheIntegrinalpha2(ITGA2)-807C／TgenepolymorphismanddiabeticnephropathyL／A0Xue—ling，CHENJun-ning，LIXiao—li，DEGNGTe，DENGYi—lan，XIEXu．DepartrnentofNephrology，theAfiliatedHospitalofGuilinMedicalCollege，Guili

4、n541001，ChinaCorrespondingauthor：CHENJun-ningE—mail：cjn3377@163．com【Abstract】0bjectiveToexploretherelationbetweentheIntegrinMpha2(ITGA2)一8o7C／Tgeneanddiabeticnephropathy(DN)inHannationalityinnorthernpartofGuangxi．Methods206patientswithtype-2diabeteswereselectedfromthehospita1．Accordingtothediag

5、nosisofdiabeticnephropathy(DN)standards，206patientswithtype·2diabetesweredividedintodiabeteswithoutdiabeticnephropathy(T2DM)andDNgroup，and90heMthypeoplewereselectedasnormalcontrol(NC)group．Theplasmalipidandlipoproteinlevelsweremeasuredbyroutinemethod．Andthegenotypesandallelefrequenciesdistribut

6、ionwereassessedbyPolymerasechainreaction—restrictionfragmentlengthPolymorphism(PCR-RFLP)．Resultscomparedwiththecontrolgroup，plasmatotalcholesterol(TC)，triglyeefide(TG)andlowdensitylipoprotein-cholesterol(LDL-C)levelsinDNgroupbloodweresignificantlyhigher(P<0．05)．ITGA2gene-807一c／Tgenotypedistribu

7、tionandallele~equencyinDNgroupwerestatisticallysignificant(P<0．05)．CT+'IFgenotypesandTallelefrequencyinDNgroupweresignificantlyhigherthancontrolgroup(P<0．05)．TherelativeriskforDNinsubjeetscarryingTallelewas1．72timesofthatintheCall