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《几种中药单体促进阿霉素内化协同诱导白血病细胞凋亡.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、ChineseJournalofVetefina~Medicine中国兽医杂志2014年(第5O卷)第6期55几种中药单体促进阿霉素内化协同诱导白血病细胞凋亡王长文,张秀荣,李妍(1.吉林医药学院公共卫生学院,吉林吉林132013;2.吉林医药学院药学院,吉林吉林132013;3.吉林医药学院检验学院,吉林吉林132013)摘要:为了分析3种中药单体对白血病细胞内化阿霉素和诱导细胞凋亡的影响,采用体外培养白血病细胞,以10I,~mol/L阿霉素处理,或同时加入中药单体(去甲基斑螫素,B一榄香烯和姜黄素)处理细胞。流式细胞术分析处理1
2、,2h和4h后细胞内化阿霉素的相对含量。继续培养至16h,流式细胞术分析细胞凋亡,并用免疫印迹技术分析细胞内活化Caspase-3的表达。结果3种中药单体中,去甲基斑蝥素和姜黄素具有显著促进阿霉素内化和促进细胞凋亡的效应;3-榄香烯也可增强阿霉紊诱导细胞凋亡的效应,但对阿霉素内化无显著影响;去甲基斑蝥素和姜黄素与阿霉素联合可增加活化Caspase-3水平。表明3种中药单体均可增强阿霉素诱导白血病细胞凋亡的效应,其中去甲基斑蝥素和姜黄素的增敏效应与其促进阿霉素的内化相关。关键词:流式细胞术;阿霉素;去甲基斑蝥素;姜黄素中图分类号:R7
3、33.7文献标志码:A文章编号:0529—6005(2014)06—0055—04ThreeKindsofChineseHerbsMonomerPromotingAdriamycinInternalizationandInduceLeukemiaCellApoptosisofThereKindsofChineseHerbsMonomerWANGChang—wen,ZHANGXiu—rong2,LIYan。(1.DepartmentofPublicHealth,JilinMedicalCollege,Jilin132013,China
4、;2.JilinMedicalCollegeDepartmentofPharmacy,Jilin132013,China;3.JilinMedicalCollegeDepartmentofMedicineLaboratory,Jilin132013,China)Abstract:Objective:ToanalyzetheeffectofthreekindsofChineseherbsmonomerontheintermalizationofadriamycininleu—kemiacellandtheinductionofcell
5、apoptosis.Methods:Theleukemiaceilswereculturedinthemediumof10I~mol/Ladriamycinin-vitro,ortheChineseherbsmonomer(norcantharidin,NCTD;p—elemieneandcurcumin)wereadded.TherelativecontentsofIntra-cellularadriamycinafterculturing1,2and4hrswereanalyzedwithflowcytometry.Thecel
6、lapoptosiswasanalyzedtillculturingl6hrswithfloweytometryandtheexpressionofCaspase一3Intraeellularactivatedwasanalyzedwithimmunoblottingtechnique.Re-suhs:AmongthreekindsofChineseherbsmonomer,NCTDandcurcuminhadsignificantefectsonpromotingtheintermalizationofadriamycinandt
7、heinductionofcellapoptosis;B—elemieneenhancedtheinductionofcellapoptosisbyadriamycinandhadinsignifi·cantefectontheinternalizationofadriamycin;Three1evelofCaspase-3couldbeenhancedbythecombinationofNCTD,cureuminandadriamycin.Conclusions:ThethreekindsofChineseherbsmonomer
8、havetheeffectontheinductionofleukemiacellapoptosisandtheenhancementeffectoftheNCTDandcureuminisrelatedtothepromotinge
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