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1、中风与神经疾病杂志2011年5月第28卷第5期429文章编号:10032754(2011)05042904亚低温治疗对脑缺血大鼠AQP4基因表达及血脑屏障通透性的影响121111任丽,王玉凯,李红芳,陈芸,郝永楠,徐鹏摘要:目的观察亚低温治疗对脑缺血大鼠AQP4表达和血脑屏障通透性的变化的影响,探讨亚低温减轻缺血性脑水肿的可能机制。方法线栓法制作脑缺血大鼠模型,实验分常温组、亚低温组和假手术组,分别在缺血后6h、24h、48h、72h用干湿重法测脑组织含水量,荧光法检测血
2、脑屏障通透性的改变,原位杂交检测AQP4的表达变化。结果假手术组脑组织含水量、血脑屏障通透性、AQP4表达无变化。缺血组脑组织含水量和血脑屏障通透性增加,AQP4表达上调,缺血48~72h变化最显著。相同时间点常温组脑组织含水量和血脑屏障通透性增加、AQP4的表达上调比亚低温组明显,两组之间的差异具有统计学意义(P<0.05)。结论亚低温条件下AQP4表达下调、血脑屏障通透性和脑水肿程度减轻。亚低温可能通过下调AQP4表达、减轻血脑屏障通透性,减轻缺血性脑水肿。关键词:亚低温;脑缺血;AQP4;血脑屏障
3、中图分类号:R743.3文献标识码:AInfluenceofmildhypothermiaontheexpressionofAQP4andthepermeabilityofbloodbrainbarrierafterratcerebralischemiaRENLi,WANGYukai,LIHongfang,etal.(DepartmentofNeurologyAffiliatedHospitalofJiningMedicalCollage,Jining272029,China)Abstract:
4、ObjectiveTostudytheinfluenceofmildhypothermiaontheexpressionofAQP4andthepermeabilityofbloodbrainbarrier(BBB)afterratcerebralischemia,andtodetectthemechanismsofmildhypothermiathatdecreasesthewatercontentinischemicbraintissue.MethodsCerebralischemicmodelswere
5、establishedbyoccludingunilateralmiddlecerebralarteriesofratswiththreads.Theratsweredividedintonormaltemperature(NT)group,mildhypothermic(MHT)groupandshamoperationgroup.Thebrainwatercontentwasmeasuredbythewetdryweighingmethodatthesametimes.ThepermeabilityofBB
6、BwasobservedwithfluorophotometrymethodandtheexpressionofAQP4wasmeasuredwithinsituhybridizationmethodon6h,24h,48hand72haftercerebralischemia.ResultsInshamoperationgroup,thebrainwatercontent,thepermeabilityofBBBandtheexpressionofAQP4hadnochanges.Theexpressionof
7、AQP4wasupregulated.ThebrainwatercontentandthepermeabilityofBBBinischemictissueincreased.Thechangesbecamemostobviousduring48hto72haftercerebralischemia.AndthechangesinNTweremoreseriousthanthoseinMHTgroupatthesametime(P<0.05).ConclusionsUndermildhypothermia,t
8、heexpressionofAQP4wasdownregulated,andthepermeabilityofBBBandthebrainedemadecreasedaftercerebralischemia.Oneofthemechanismsofmildhypothermiacoulddecreasethewatercontentinischemicb
